目的 测定蒙古黄芪多糖中的单糖组成。方法 蒙古黄芪多糖样品用三氟乙酸溶液水解成单糖，用1-苯基-3-甲基- 5-吡唑啉酮（PMP）衍生化，采用HPLC法于245 nm波长处检测各单糖。结果 蒙古黄芪多糖由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、阿拉伯糖组成，物质的量比分别为0.02∶0.05∶0.17∶1∶0.18。结论 此方法简单、快速、重复性好，可用于蒙古黄芪多糖中单糖组成分析。

Objective To determine the monosaccharide compositions in Astragalus mongholicus var. mongholicus polysaccharides (AMMP). Methods AMMP samples were first hydrolyzed with trifluoroacetic acid, and then derivatized with 1-phenyl-3-methyl-5- pyrazolone (PMP). Monosaccharide was determined by HPLC at detective wavelength of 245 nm. Results AMMP composed of mannose, rhamonse, galacturonic acid, glucose, and arabinose with molar ratio 0.02∶0.05∶0.17∶1∶0.18. Conclusion The method is simple, fast, and reproducible, and could be used for the analysis of monosaccharide compositions in AMMP.

国家“十二五”科技支撑计划资助项目（2011BAI07B01）