目的 优化北苍术多糖的超声提取工艺。方法 利用超声波提取北苍术根茎中的多糖，以提取物得率和多糖提取率为指标，在单因素试验的基础上，采用4个因素（超声时间、料液比、超声温度、乙醇用量）3个水平的L9(34)正交试验优选超声提取工艺条件，并采用苯酚–浓硫酸法测定多糖。结果 北苍术多糖的最佳提取工艺条件为：料液比1∶20、提取温度60 ℃、超声提取40 min、浓缩后加入4倍量乙醇进行醇沉。在该实验条件下，苍术水提物得率为23.16%，多糖提取率为11.23%。结论 超声法提取苍术中多糖简便快捷，多糖提取率高，为北苍术多糖的开发利用提供了参考。

Objective To optimize the extraction technology of polysaccharide from Atractylodes chinensis using ultrasonic extraction method by orthogonal test. Methods Polysaccharide was extracted from A. chinensis by ultrasonic-assisted extraction method. The yield of crude polysaccharide of A. chinensis and extraction rate of polysaccharide were taken as indices. Based on single-factor experiment, the ultrasonic-assisted extraction technology of polysaccharide from A. chinensis was optimized by L9(34) orthogonal test. The phenol-sulphuric acid method was applied for the determination of polysaccharide. Results The optimal extraction conditions of polysaccharide from A. chinensis were as follows: the solid-liquid ratio was 1∶20; the extraction temperature was 60 ℃; the extraction time was 40 min and alcohol volume was 4 times of the medicinal materials. Under the conditions, the yield crude polysaccharide and the purity of polysaccharide were 23.16% and 11.23%, respectively. Conclusion Ultrasonic extraction has the advantages of short time, simple operation, and higher extract rate, which could provide the reference for development and utilization of A. chinensis.

天津市科技攻关项目（06YFGPSH02900）