目的 建立高效液相色谱–蒸发光散射检测器（HPLC-ELSD）法测定安多霖胶囊中黄芪甲苷的方法。方法 采用Ultimate色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈–超纯水（32∶68），体积流量为1 mL/min，漂移管温度40 ℃，蒸发光散射检测器雾化气体为氮气，体积流量为2.7 L/min，载气压力350 kPa，进样量20 μL。结果 黄芪甲苷在1.59～10.60 μg呈良好的线性关系，r=0.999 5，平均回收率为99.11%，RSD为2.58%（n=6）。结论 本方法简便准确，重现性好，可用于安多霖胶囊的质量控制。

Objective To develop a method for the determination of astragaloside in Anduolin Capsule by HPLC-ELSD. Methods The samples were separated on Ultimate column (250 mm×4.6 mm, 5 μm) with acetonitrile-water (32:68) as mobile phase at a flow rate of 1 mL/min. The temperature of drift tube for measurement was 40 ℃, and the flow pressure of N2 was 350 kPa with injection volume of 20 μL at a flow rate of 2.7 L/min. Results The linear range of astragaloside was 1.59―10.60 μg (r=0.999 5). The average recovery rate was 99.11% with RSD at 2.58% (n=6). Conclusion This method is simple, accurate and repeatable for the quality control for Anduolin Capsule.