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[摘要]
目的 采用正交试验优化活性炭对白芷多糖提取液脱色的工艺条件。方法 考察脱色温度、活性炭添加量和脱色时间对白芷多糖脱色效果的影响。在单因素试验的基础上,以多糖脱色率和多糖剩余率为指标,采用L9(34)正交试验确定了最优工艺的参数。结果 活性炭添加量对脱色效果的影响最大,其次为温度,再次为时间。最佳脱色条件为脱色温度60 ℃,活性碳添加量2%,吸附时间30 min,在此条件下,脱色率为96.20%,多糖剩余率为82.36%。结论 活性炭对白芷多糖脱色工艺简便可行。
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[Abstract]
Objective To optimize the decoloration technology for active carbon on polysaccharides from Angelicae Dahuricae Radix by orthogonal test. Methods Effects of decoloration temperature, amounts of active carbon, and decoloration time on polysaccharides from Angelicae Dahuricae Radix were investigated. Based on the single factor test method, L9(34) orthogonal test method was performed to optimize the technological paramters with decoloration ratio and the remaining rate of polysaccharide as indexes. Results The sequence of the effects on the decoloration was amount of active carbon, temperature, and time. The optimized decoloration conditions were decolorating temperature 60 ℃, the amount of active carbon 2%, and decolorating time 30 min. Under this condition the decoloration rate was 96.20% and the remaining rate of polysaccharide was 82.36%. Conclusion The process of the decoloration technology for active carbon on polysaccharides from Angelicae Dahuricae Radix is reasonable and feasible.
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