目的 采用高速逆流色谱法分离制备多花蒿中阿格拉宾。方法 多花蒿乙醇提取物经硅胶柱色谱分离，所得组份Fr. 1用高速逆流色谱进一步分离，采用正己烷–醋酸乙酯–甲醇–水（1.2∶0.8∶1.5∶0.5）为两相溶剂系统，上相为固定相，下相为流动相，主机转速850 r/min，体积流量2.0 mL/min，检测波长为254 nm。采用波谱法对所得化合物进行结构鉴定，HPLC法测定产品的纯度。结果 从500 mg组分Fr. 1中得到260 mg质量分数为99.5%的阿格拉宾。结论 该方法操作简单，可用于阿格拉宾化学对照品的分离制备。
Objective To establish a method for the isolation and preparation of arglabin from Artemisia myriantha by high speed counter-current chromatography (HSCCC). Methods The ethanol extract of A. myriantha was separated with silica gel column chromatography, and the obtained Fr. 1 was further separated by HSCCC with a two-phase solvent system composed of n-hexane-ethylacetate-methanol-water (1.2:0.8:1.5:0.5). The lower phase was used as mobile phase, and the upper phase was used as the stationary phase. Main engine speed was 850 r/min, and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min. The obtained fraction was identified by spectral analysis and analyzed by HPLC. Results A total of 260 mg arglabin with the purity of 99.5% was successfully obtained from 500 mg Fr. 1. Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of arglabin reference substance.