目的 建立无糖型宁心安神糖浆中五味子醇甲和五味子乙素的测定方法。方法 采用Kromasil C18色谱柱（250 mm×4.6 mm，5 μm），流动相：甲醇–水（73∶27），检测波长：250 nm，柱温：30 ℃，体积流量：1.0 mL/min，进样体积：10 μL。结果 五味子醇甲、五味子乙素分别在0.16～1.6 μg（r=0.999 9）和0.04～0.40 μg（r=0.999 1）呈良好的线性关系，平均加样回收率分别为100.00%、100.04%，RSD分别为0.60%、0.18% （n=6）。结论 该方法简便、准确、重现性好，可作为无糖型宁心安神糖浆质量控制的方法。

Objective To develop a method for the determination of schisandrol A and schisandrin B in Sugarless Ningxin Anshen Syrup. Methods The samples were separated on Kromasil C18 column (250 mm×4.6 mm, 5 μm) with methanol -water (73:27) as mobile phase. The detection wavelength was 250 nm and column temperature was 30 ℃ with injection volume of 10 μL at a flow rate of 1.0 mL/min. Results The calibration curve was linear over the range of 0.16 —1.6 μg (r=0.999 9) and 0.04 — 0.40 μg (r=0.999 1) for schisandrol A and schisandrin B, respectively. The average recovery rate was 100.00% and 100.04% with RSD at 0.60% and 0.18%, respectively (n=6). Conclusion This method is simple, accurate and repeatable for the quality control of Sugarless Ningxin Anshen Syrup.