目的 建立替米沙坦中有关物质的测定方法。方法 采用反相高效液相色谱（RP-HPLC）法，使用Dikma Diamonsil C18色谱柱（150 mm×4.6 mm，5 μm），[磷酸二氢钾–戊烷磺酸钠缓冲液（pH 3.0）]–[甲醇–乙腈（20∶80）]线性梯度洗脱，检测波长为230 nm，体积流量为1.0 mL/min，柱温为35 ℃。结果 在上述色谱条件下，5种杂质能与替米沙坦有效地分离，分离度大于1.5，检出限为0.1 ng。结论 使用本方法测定替米沙坦中有关物质，能有效检出特定杂质，方法准确、快速、重复性好。

Objective To establish a method for the determination of related substances in telmisartan. Methods The RP-HPLC method was carried out using a Dikma Diamonsil C18 column (150 mm×4.6 mm, 5 μm), the mobile phase consisted of KH2PO4-pentane sulfonic acid sodium salt buffer (pH 3.0) and methanol-acetonitrile (20∶80) with linear gradient elution, the detective wavelength was at 230 nm with the flow rate of 1.0 mL/min and the temperature at 35 ℃. Results Five impurities were effectively separated from telmisartan with separating degree (R)>1.5, and detection limit 0.1 ng. Conclusion The related substances could be determined from telmisartan by the method, and this method with better reproducibility is precise and rapid.