目的 参比商品珍珠粉及珍珠蚌壳粉液相指纹特征，建立淡水养殖1～5年珍珠中氨基酸的液相指纹图谱以控制质量。方法 采用HPLC测定，色谱条件：Venusil-AA氨基酸分析柱，乙腈–0.1 mol/L的醋酸钠水溶液（pH 6.50）为流动相，梯度洗脱，检测波长254 nm，体积流量1.0 mL/min，柱温45 ℃。结果 建立了HPLC指纹图谱共有模式，得出不同品系的样本相似度分布谱带。结论 色谱指纹图谱分析法能准确地鉴别区分不同品系的珍珠类产品，可为珍珠粉的质量控制和真伪鉴别提供依据和方法。

Objective To set up the HPLC fingerprints of amino acids in frreshwater aquaculture pear powder for 1－5 years and compare the fingerprints with that of commercial pearl powder and of pearl shell powder so as to establish a sensitive and specific method. Methods The HPLC separation was performed on a Venusil-AA analytical column (commercial spectrum column special for analyzing amino acids) and by gradient elution with acetonitrile-0.1 mol/L sodium acetate solution (pH 6.5) at the flow rate of 1.0 mL/min. The temperature of column was 45 ℃. The detection wavelength was 254 nm. Results The common mode of HPLC fingerprints was set up and the similar degrees of commercial pearl powder and pearl shell powder to crude pearl powder were compared. Conclusion It is simple and rapid to identify and differentiate pearl powder collected from different sources with the method which can be used as a quality control item for pearl powder and its related products.