目的 观察散结镇痛片对子宫内膜异位症模型大鼠妊娠功能和雌激素水平的影响。方法 采用自体种植法制备大鼠子宫内膜异位症模型。手术后将大鼠分为假手术组，模型组，达那唑胶囊（0.070 g/kg）阳性对照组，散结镇痛片低、中、高剂量（0.216、0.432、0.864 g/kg）组及散结镇痛胶囊（0.864 g生药/kg）组，每天ig给药1次，连给4周后，大鼠眼眶静脉丛取血，放射免疫法测血清中促黄体生成素、催乳素和孕激素的水平。取各组大鼠与有生育能力的雄鼠合笼交配，1周后将雄鼠取出，分笼饲养，于分笼后第12天处死雌鼠，摘取右卵巢，计数妊娠黄体数，剖开右宫角，计数着床点、活胎及死胎数，并计算着床率和死胎率。结果 与模型组比较，散结镇痛片3个剂量均能增加活胎数、排卵数，提高着床率，降低死胎率，其中高剂量增加排卵数，提高着床率的作用尤为明显；散结镇痛片3个剂量均能调节大鼠血清催乳素和孕激素水平，其中高剂量降低催乳素、升高孕激素水平的作用尤为明显。结论 散结镇痛片能增强子宫内膜异位症模型大鼠的妊娠功能，该作用可能与其调节激素水平、恢复机体正常内分泌功能有关。

Abstract: Objective To observe the effects of Sanjie Zhentong Tablet on pregnant function and estrogen level of rats with experimental endometriosis. Methods Self-endometrium transplantation by surgical method was used to develop rats models of endometriosis. After operation, the rats were divided into seven groups, such as Sham group, model group, Danazuo (0.070 g/kg) group, high, medium, and low dosage (0.216、0.432、0.864 g/kg) of Sanjie Zhentong Tablet group, and Sanjie Zhentong Capsula (crude drugs 0.864 g/kg) group. Four weeks after ig administration once daily, blood samples were drawn from orbital venous plexus of rats, the serum LH, P and PRL levels were measured by radioimmunoassay. Then the rats were mated with known fertile male rats, that were removed after one week, sub-cage feeding, 12 d later, females were killed to open the abdominal cavity, then remove the right ovary, count the number of corpus luteum of pregnancy, cut and open the right uterine horn, count the implantation sites, the number of live births and stillbirths, and calculate the implantation rate and stillbirth rate. Results Three doses of Sanjie Zhentong Tablet can increase the number of live births, ovulation rate, and the implantation rate, while reduce the stillbirth rate, among which high dose of Sanjie Zhentong Tablet could significantly increase the number of ovulation rate and implantation rate；all the three doses of Sanjie Zhentong Tablet are able to regulate the level of serum PRL and P, in which large doses can significantly reduce the serum levels of PRL, elevate P level. Conclusion Sanjie Zhentong Tablet can improve the pregnant functions of experimental endometriosis in rats, the mechanism may be related to its functions in regulating hormone levels and restoring the body's normal endocrine.

国家重大新药创制专项（2011ZX092012-066）