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[摘要]
探讨肉桂醛抗烟曲霉的作用机制。方法 采用固体插片培养法培养烟曲霉,定时取样品于光学显微镜下动态观察烟曲霉生长情况,以RT-PCR检测在肉桂醛作用下烟曲霉FKS基因表达的变化。结果 以肉桂醛0.025 μg/mL作用后,烟曲霉生长显著迟缓,分生孢子头稀疏;烟曲霉FKS基因的表达受到抑制,但随培养时间的延长,该抑制作用减弱。结论 肉桂醛通过抑制细胞壁主要多糖合成的关键基因FKS表达,使烟曲霉细胞壁失去正常生理结构,细胞生长、繁殖被抑制,从而达到抗烟曲霉的目的。
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[Abstract]
To discover the anti-aspergilli mechanism of cinnamaldehyde on Aspergillus fumigatus. Method Using Czapak's solid petri dish containing glass coverslip, its growth and propagattion were observed timely by light microscope, RT-PCR was performed to detect the effect of cinnamaldehyde on gene expressions of FKS. Results A. fumigatus treated by cinnamaldehyde at concentration of 0.025 μg/mL showed obviously slow growth rate and markly reduced conidial head produced; cinnamaldehyde could inhibit the gene expressions of FKS, and this effect became weaker over incubation time. Conclusion Cinnamaldehyde could inhibit the gene expression of key gene FKS and damage the cell wall structure, which and would be one of its anti-aspergilli mechanism.
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