目的 建立一种HPLC法快速测定北冬虫夏草和几种常见食用菌中腺苷和虫草素的方法。方法 Welch Material XB-C18色谱柱（250 mm×4.6 mm， 5 μm），以水–甲醇（85︰15）为流动相，体积流量1.0 mL/min，检测波长258 nm，柱温40 ℃。结果 腺苷在0.023 2～0.464 0 μg、虫草素在0.030 0～0.600 0 μg与峰面积线性关系良好，平均加样回收率分别为98.97%、99.15%，RSD分别为1.41%、1.01%。结论 腺苷普遍存在于各种食用菌子实体中，而虫草素是虫草属真菌子实体中的特有活性成分。本法简便快速，为全面评估北冬虫夏草中核苷类成分的质量，推进人工培育北冬虫夏草替代野生冬虫夏草提供检测方法。

Objective To develop a rapid method by using HPLC to quantitatively determine adenosine and cordycepin in Cordyceps militaris products and common edible fungi. Methods The working conditions: column was Welch Material XB-C18 column (250 mm×4.6 mm, 5 μm), mobile phase was composed of water-methanol (85︰15) with flow rate of 1.0 mL/min and 258 nm detection wavelength, and temperature was 40 ℃. Results The linearity range of adenosine and cordycepin were 0.023 2—0.464 0 and 0.030—0.600 μg. The average recovery of adenosine and cordycepin were 98.97% and 99.15% with the RSD of 1.41% and 1.01%, respectively. Conclusion Adenosine appears widely in common edible fungi, and cordycepin is characteristic in fruitbodies of Cordyceps (Fr.) Link. The HPLC method is simple and fast., and could lay a method on the comprehensive evaluation of the nucleoside components in C. militaris, and promot the cultured C. militaris to replace the natural C. sinensis resources.

国家863计划重点项目（2007AA021502）