Pine needle is the leaves of Pinaceae plant. It mainly contains volatile oil, flavonoids, lignans, pigment, vitamin, amino acids, and many minerals. Pine needle’s application for medicinal purposes has a long history. Modern research suggests pine needle has many pharmacological effects such as fall in the blood sugar and blood fat, inhibititng tumor, anti-aging, antimutation, antibacterial, antiviral and analgesia. The recent research about chemical constituents and its pharmacological activities of pine needles both at domestic and abroad have been summarized in this paper, expect for providing a reference for the further study, exploitation, and utilizing of this resource.