目的 建立马钱子散中士的宁的高效液相色谱测定方法。方法 采用Akasil-C18柱（200 mm×4.6 mm，5 μm），以甲醇–0.01mol/L磷酸二氢钾溶液（25∶75）为流动相，体积流量为1.0 mL/min，检测波长为254 nm。结果 士的宁进样量在0.48～2.4 μg与峰面积线性关系良好（r＝0.999 5），平均回收率为98.9%，RSD为2.2%（n＝6）。结论 所建立的方法专属性强、准确、快速，适用于马钱子散中士的宁的测定。

Objective To develop a reversed-phase HPLC method for analyzing the content of strychnine in Maqianzi Powder. Methods The HPLC system comprised of an Akasil-C18 column (200 mm×4.6 mm, 5 μm). The mobile phase was a mixture of methanol and 0.01 mol/L potassium dihydrogen phosphate solution (25∶75) and delivered at a flow rate of 1.0 mL/min. The detection wavelength was done at 254 nm. Results The calibration curve was found to be linear in injection amount range of 0.48—2.4μg (r = 0.999 5). The mean recovery of strychnine was 98.9 % (RSD = 2.2%, n = 6). Conclusion These results indicate that the HPLC method could be suitable for monitoring of strychnine in Maqianzi Powder since it is selective, accurate, and rapid.