目的 建立测定青龙衣中羟基萘醌的方法，考察不同贮藏年限的青龙衣药材中羟基萘醌的变化。方法 以胡桃醌为对照品，5%氢氧化钾为显色剂，在515 nm波长下，采用比色法测定青龙衣中羟基萘醌，对不同贮藏年限的青龙衣药材中羟基萘醌进行比较。结果 贮藏年限为一年、二年和三年的青龙衣药材中羟基萘醌的量存在明显差异，即随着贮藏时间的延长，青龙衣中羟基萘醌的量逐渐下降。结论 青龙衣药材不宜储藏时间过长，尽量应用当年的药材。

Objective To establish a determination method of hydroxyl naphthoquinones for Qinglongyi (fresh rejuvenated fruits of Juglans mandshurica), and to compare hydroxyl naphthoquinones from Qinglongyi in different storage times. Methods Juglone was used as standard, 5% KOH solution was used as chromogenic reagent, and wavelength was at 515 nm. Hydroxyl naphthoquinones in Qinglongyi in different storage times were determined by colorimetric method and compared. Results There were great differences among hydroxyl naphthoquinones contents of storage period 1, 2 and 3 years of Qinglongyi. The hydroxyl naphthoquinones contents of Qinglongyi gradually decreased with the storage time prolonged. Conclusion Qinglongyi should not be kept too long time, and should be used that very year.

国家自然科学基金资助项目（30600816）；高等学校博士点学科点专项科研基金资助项目（20060240001））