甘草因疗效显著、药理作用明确，一直以来都是研究的热点。近年来有20个黄酮类化合物和6个三萜类化合物被陆续分离出来，其中包括16个新的黄酮类化合物和3个新的三萜类化合物。细胞培养是获得甘草有效成分的有效途径之一，目前应用细胞培养技术主要生产甘草黄酮类成分。综述了近年来从甘草中提取的黄酮类、三萜类化合物，细胞培养方法对甘草黄酮类成分的影响，以及毛细管电色谱法、HPLC法、免疫测定法等检测甘草化学成分方法的研究进展。

Glycyrrhizae Radix et Rhizoma has been the research focus because of its conspicuous healing efficacy and pharmacological activities. Recently, 20 flavonoids and 6 triterpenes compounds, including 16 new flavonoids and 3 new triterpenes have been isolated from this plant. It was also found that cell culture provided an effective way to acquire effective components of Glycyrrhizae Radix et Rhizoma. The cell culture technique is mainly used for product Glycyrrhizae Radix et Rhizoma flavonoids. In this paper, the recent progresses was reported on the chemical components of the plant, the cell culture method for the bioactive flavonoids ingredients and determination of its effective constituents using capillary electrochromatography, HPLC, and immunoassays detection as well.

“十一?五”国家科技重大专项资助项目（2009ZX09501-011）；新疆维吾尔自治区科学技术厅资助项目（200915122）