目的 建立苦杏仁中苦杏仁酶的闪式提取工艺，并对其工艺条件进行优化。方法 以苦杏仁酶总活力为指标，考察了氨水浓度、提取液用量、提取时间、中和终点pH值和沉淀时乙醇体积分数对闪式提取工艺的影响；采用二硝基水杨酸（DNS）法测定苦杏仁酶的比活力，并计算其总活力。结果 苦杏仁酶的最佳提取工艺条件为：取50.0 g脱脂苦杏仁粉，使用500 mL 62.5 mmol/L预冷氨水提取120 s 后，残渣再使用450 mL提取液提取90 s，滤过，滤液用36%醋酸调pH 4.0，滤去杂蛋白后加入4倍量乙醇，沉淀苦杏仁酶。结论 采用闪式提取法改进了苦杏仁酶的提取工艺，并对该工艺进行了优化，得出了较优的闪式提取条件。该工艺具有周期短、操作简便、酶活力高等优点，提取效果优于传统方法。

Objective To establish and optimize the extraction technology of emulsin from Armeniacae Amarum Semen using smashing tissue extraction method. Methods These factors, such as ammonia concentration, the amount of extract, extraction time, terminal point pH value of counteraction, and the volume fraction of ethanol for precipitation influencing on the extraction process of smashing tissue extraction were investigated by using total enzymatic activity of emulsin as index. The specific enzymatic activity of emulsin was determined by dinitrosalicylic acid (DNS) method, and the total enzymatic activity was calculated. Results The optimum extraction conditions were as following: 50.0 g powder of defatted Armeniacae Amarum Semen was extracted with 500 mL 0.062 5 mol/L pre-cooling ammonia for 120 s. The mixture was filtrated by gauzes and the residue was extracted with 450 mL extract for 90 s again. Collected the filtrate, adjusted its pH value to 4.0 by acetic acid, filtered hybrid protein, and precipitated the emulsin by four times the amount of ethanol. Conclusion The smashing tissue extraction is used to improve the extraction technology of emulsin from Armeniacae Amarum Semen for the first time, and the conditions of the new technology are optimized by determining the specific activity of emulsin. The results show that smashing tissue extraction has the advantages of short time, simple operation, and higher enzymatic activity, which is better than traditional extraction method.