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[摘要]
目的 研究大孔吸附树脂对枳实中辛弗林的吸附性能,考察精制工艺。方法 以辛弗林质量分数和收率为考察指标,比较了HPD-300、NKA-9、AB-8、D001-cc等树脂对枳实中辛弗林的吸附和解吸特性,以及纯化能力。结果 D001-cc树脂具有最佳的吸附洗脱参数,上样液生药质量浓度为0.5 g生药/mL,4倍体积蒸馏水、2倍体积1.5 mol/L氨水依次洗脱,产物中辛弗林质量分数为46.3%。得率为1%。结论 D001-cc树脂对枳实中辛弗林综合性能较好,适合于辛弗林的分离精制。
[Key word]
[Abstract]
Objective To optimize the technology for purification of synephrine in Aurantii Immaturus Fructus by resins. Methods HPD300, NKA-9, AB-8, and D001-cc resins were used to separate and purify synephrine. The yields and purities of the products were compared as indexes. Results D001-cc type macroporous ion resin had optimum adsorption and elution parameters. The appropriate adsorption conditions were: the concentration of crud drug was 0.5 mg/mL, flow rate was 1 BV/h, the effect of desorption was satisfactory when 1.5 mol/L ammonia (2 BV) and water (4 BV) were used as eluant. Conclusion The D001-cc type macroreticular resin shows a better comprehensive adsorption property and can be used to separate and purify synephrine in Aurantii Immaturus Fructus.
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[基金项目]
天津市自然科学基金资助项目(10JCZDJC21400);天津市科技支撑计划资助项目(08ZCKFCG00200)