摘 要：目的 建立高效液相色谱法测定米诺膦酸片中米诺膦酸。方法 采用安捷伦XDB-C18色谱柱（250 mm×4.6 mm，5 μm），流动相为0.03 mol/L焦磷酸钠水溶液（含四丁基溴化铵0.322 g/L，以磷酸调节pH 7.5）-甲醇（85∶20），体积流量1 mL/min，柱温30 ℃，检测波长280 nm，进样量20 μL。结果 米诺膦酸在0.02～0.18 mg/mL时，峰面积与质量浓度呈良好的线性关系（r=0.999 9）。结论 该方法准确、可靠，可作为米诺膦酸片的测定方法。

Abstract: Objective To establish a RP-HPLC method for the determination of minodronic acid in Minodronic Acid Tablets. Method The Agilent column XDB-C18 (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisited of 0.03 mol/L sodium pyrophosphate (0.322 g/L tetrabutylammonium bromide, adjusting pH value to 7.5 with phosphorous acid)-methanol (85∶20). The flow rate was 1.0 mL/min with the column temperature at 30 ℃. The detection wavelenth was 280 nm and the injection volumn was 20 μL. Results The solution of minodronic acid was stability. The linear range for minodronic acid was 0.02—0.18 mg/mL (r=0.999 9). Conclusion The method is accurate and reliable, which is suitable for the determination of Minodronic Acid Tablets.