摘 要：目的 更好地控制调经助孕颗粒剂的质量。方法 采用 TLC 法对调经助孕颗粒剂中的白芍、川楝子进行定性鉴别；HPLC 法对柚皮苷进行定量检测，色谱柱为 Symmetry C18（150 mm×2.1 mm，3.5 μm），流动相为乙腈–0.1%磷酸溶液（18∶82），体积流量0.8 mL/min，柱温35 ℃，检测波长283 nm。结果 TLC 显色清晰；柚皮苷在0.404～5.05 μg 有良好线性关系（r＝0.999 3），平均回收率为99.26%，RSD 为2.03%。结论 本研究可用于调经助孕颗粒剂的质量控制，方法简便、可靠，能较好地控制该制剂的内在质量。

Abstract: Objective To control effectively the quality of Tiaojing Zhuyun Granules．Methods Paeoniae Radix Alba and Toosendan Fructus in this medicine were identified by TLC．Naringin was determined by HPLC，using Symmetry C18 column with a mixture of acetonitrile-0.1% phosphoric acid（18∶82）as the mobile phase. The temperature of column was 35 ℃．The velocity was 0.8 mL/min．The detection wavelength was 283 nm. Results TLC spots were quite clear．There was a good linear relationship at a range of 0.404—5.05 μg（r＝0.999 3）．The average recovery rate was 99.26%，and RSD was 2.03%．Conclusion The method is simple，reliable and reproducible．It can be used for the quality control of Tiaojing Zhuyun Granules．

黑龙江省中医管理局研究课题（ZHY08-W101）