摘 要：目的 首次以藁本内酯纯度和转移率为主要考察指标，探讨应用超临界 CO2 萃取法从当归中提取总苯酞的工艺。方法 采用单因素和 L9(3)4 正交设计进行试验，考察药材粒度、萃取压力、萃取时间、萃取温度等因素对藁本内酯纯度和转移率的影响，优化最佳提取工艺。采用高效液相色谱法分析，色谱条件为 Accurasil C18 柱（250 mm×4.6 mm，5 μm），流动相为甲醇-0.05%磷酸水溶液，柱温30 ℃，检测波长320 nm，体积流量1.0 mL/min。结果 超临界 CO2 萃取法的最佳工艺条件为：萃取压力10 MPa，萃取温度50 ℃，萃取时间20 min。结论 超临界 CO2 流体工艺萃取的当归总苯酞纯度和转移率可达80%以上。

Abstract: Objective To establish the optimal supercritical CO2 extraction of total phthalide from Angelica sinensis．The ligustilide purity and transfer rate in general phthalide were used as the evaluation indexes．Methods The optimum extraction conditions，such as sizes of crush materials，the extraction pressure，extraction temperature，and extraction time were investigated by L9(3)4 orthogonal test．The HPLC was performed on a Accurasil C18 column（250 mm×4.6 mm，5 μm）．The mobile phase consisted of methanol-0.05% phosphoric acid water．The column temperature was 30 ℃．Examination wavelength was 320 nm．The flow rate was 1.0 mL/min. Results The optimal supercritical CO2 extraction conditions were: extraction pressure，10 MPa；extraction temperature，50℃；extraction time，20 min．Conclusion It is feasible that supercritical CO2 extraction could be used in the extraction of total phthalide from A.sinensis，its purity and transfer rate are over 80%．

“十一五”国家科技支撑计划课题“当归、黄芪、大黄等区域特色中药资源利用关键技术及产业化研究”子课题（2007BAI37B06）