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[摘要]
摘 要:目的 建立健儿消食口服液中橙皮苷和黄芩苷的定量测定方法。方法 采用 HPLC 法,色谱柱为 Agilent Eclipse XDB(250 mm × 4.6 mm,5 μm),流动相为乙腈-0.3%磷酸溶液(23︰77),检测波长283 nm。结果 橙皮苷在0.090 25~0.722 0 μg 呈良好的线性关系;黄芩苷在0.323 25~2.602 0 μg 呈良好的线性关系。平均回收率分别为99.62%、98.99%;RSD 分别为1.86%、1.66%。结论 本方法简便、准确、重现性好,可用于健儿消食口服液的质量控制。
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[Abstract]
Abtract: Objective To establish a method for the simultaneous determination of hesperidin and baicalin in Jian′er Xiaoshi Oral Liquid.Methods RP-HPLC was adopted with Agilent Eclipse XDB(250 mm × 4.6 mm,5 μm)as analytical column.The mobile phase was acetonitrile - 0.3% phosphoric acid solution(23︰77).The detection wavelength was at 283 nm.Results The linear range of hesperidin was 0.090 25—0.722 0 μg,the average recovery was 99.62% and RSD was 1.86%.The linear range of baicalin was 0.323 25—2.602 μg,the average recovery was 98.99%,and RSD was 1.66%.Conclusion The method is convenient,accurate and reproducible,and can be applied to quality control of Jian′er Xiaoshi Oral Liquid.
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