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[摘要]
摘 要:目的 探讨 N-硬脂酰酪氨酸(NsTyr)对缺氧缺糖(OGD)诱导大鼠皮层神经元损伤的影响及作用机制。方法 采用 MTT 法及 Hoechst 33342染色检测 NsTyr 对 OGD 诱导神经元损伤及凋亡的影响;通过免疫印迹法探讨 NsTyr 抗 OGD 诱导神经元凋亡的分子机制,并使用丝裂原活化蛋白激酶(MAPK)通路的特异性阻断剂 SB203580、SP600125和 U0126考察其抗凋亡的信号转导通路。结果 NsTyr 对 OGD 造成的皮层神经元损伤有剂量相关的保护作用,促进细胞存活,减少细胞凋亡;NsTyr 通过上调 Bcl-2表达、下调 Bax 表达、保持 Bcl-2/Bax 的平衡,实现抗 OGD 损伤的作用,该作用通过 ERK 和 p38信号通路介导。结论 NsTyr 可通过 ERK 和 p38信号通路调节凋亡基因的表达,抑制 OGD 引起的神经元凋亡。
[Key word]
[Abstract]
Abstract: Objective To investigate the protective effects of N-stearoyltyrosine(NsTyr)against OGD-induced apoptosis on primary cultured rat′s cortical neurons.Methods Cell death was evaluated with MTT assay,and the neuron apoptosis was analyzed through Hoechst staining.The mechanism and the signal pathways involved were studied by western blot analysis and the treatment of specific MAPK inhibitors.Results NsTyr alleviated does-dependently the OGD-induced toxicity to cortical neurons.The protection of NsTyr was through reversing the up-regulation of Bax and down-regulation of Bcl-2.Moreover,the effect was blocked by SB203580 and U0126,while no obvious influence on the regulation of Bcl-2 by SP600125 was observed.Conclusion NsTyr possesses protective effects on cortical neurons by regulating the balance between Bax and Bcl-2 through ERK and p38 signaling pathways.
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[基金项目]
国家自然科学基金资助项目(30672441,30873057);上海市科委基础重点课题(08JC1413600);十一五“重大新药创制”科技重大专项“综合性新药研究开发技术大平台”(2009ZX09301-007)