目的 建立RP-HPLC法同时测定女贞叶乙醇提取物中齐墩果酸与熊果酸的方法。方法 通过系统地考察温度、pH值、甲醇-乙腈混合有机相中甲醇的量对齐墩果酸、熊果酸保留时间和分离度的影响，最终确定HPLC色谱条件为大连依利特Kromasil C18（250 mm×4.6 mm，5 μm）,色谱柱；甲醇-乙腈-10 mmol/L乙酸铵(55∶27∶18)为流动相，体积流量为1 mL/min，检测波长为205 nm，柱温20 ℃。结果 齐墩果酸与熊果酸分别在0.300～300 μg、0.692～6.92 μg线性关系良好；加样平均回收率分别为101.4%、100.2%(n=9)。结论 低温、中性pH、甲醇-乙腈混合有机相有利于齐墩果酸与熊果酸的分离。优化后的色谱方法分离度高，重现性好，可用于女贞叶乙醇提取物中齐墩果酸与熊果酸的测定。

Objective To establish a RPHPLC method for simultaneous quantitative determination of oleanolic acid and ursolic acid in ethanol extract of Ligustrum lucidum Aitleaves.Methods Temperature，pH value and proportion of methanol and acetonitrile in organic phase were systematically studied on the influence of retention and resolution between oleanolic acid and ursolic acid. The optimized chromatographic condition was Kromasil C18 column (250 mm×4.6 mm, 5 μm);methanolacetonitrile 10 mmol/L ammonium acetate (55∶27∶18) as mobile phase with flow rate 1.0 mL/min. The detection wavelength was 205 nm and col umn temperature was 20 ℃.Results The linear ranges of oleanolic acid and ursolic acid were 0.300～3.00 μg，0.692～6.92 μg respectively; the average recoveries were 101.4%， 100.2%(n=9) respectively.Conclusion Low temperature，neutral pH and mixed organic phase with methanolacetonitrile can improve resolution of oleanolic acid and ursolic acid. The optimized method can achieve better resolution and reproducibility，and is suitable to determine the content of oleanolic acid and ursolic acid in ethanol extract of Ligustrum lucidum Ait leaves.