目的 探讨与地塞米松磷酸钠注射液合用时注射用双黄连中绿原酸在大鼠体内血药浓度及相关药动学参数的影响。方法 24 只SD系大鼠，雌雄各半，随机分成2 组，尾静脉给药后采用反相高效液相色谱法，测定单独给予注射用双黄连及与地塞米松合用后不同时间点的大鼠体内绿原酸血药浓度，并对所得数据用DAS2.0软件进行分析，求出其主要药动学参数。结果 静脉给药后, 绿原酸在大鼠体内符合二室开放模型，在单用和复合给药时的分布半衰期(,i>t_1/2α)、总消除率(Cl)和药-时曲线下面积(AUC)等主要药动学参数均有显著性差异。结论 在给药剂量下，单用和合用的血药浓度变化有较大差异；两者合用时，地塞米松对绿原酸的药动学有显著影响，促进大鼠体内绿原酸的消除，从而加速双黄连的代谢。

Objective To study the concentration and pharmacokinetics parameters of chlorogenic acid in rats after intravenous injection of Shuanghuanglian only or Shuanghuanglian combined with Dexamethasone Sodium Phosphate Injection. Methods A total of 24 SD rats were randomly divided into two groups: single injection group and combination injection group. RP-HPLC method was used to determine the concentrations of chlorogenic acid in rats plasm at different time after intravenous injection of Shuanghuanglian- an only or Shuanghuanglian combined with Dexamethasone. Pharmacokinetic model and parameters were analyzed by Drug and Statistics (DAS 2.0). Results It was two-compartment model in rats. There were significant differences in the main pharmacokinetics parameters such as t_1/2α，Cl and AUC between the single injection group and the combination injection group. Conclusion Dexamethasone may change remarkable plasma concentration of chlorgenic acid. There is evidence of pharmacokinetic interaction between Dexamethasone and chlorogenic acid after the intravenous injection in rats. Dexamethasone can enhance the metabolism of Shuanghuanglian.

国家自然科学基金资助项目(20905015)；广东省自然科学基金资助项目(9451022401003240)；广东省科技计划资助项目(2008B030301035)；广东省中医药局资助项目(2009248)