目的 建立藏药独一味中类叶升麻苷的测定方法。方法 采用HPLC法。色谱条件：Diamonsil C18色谱柱（200 mm×4.6 mm, 5 μm）；以乙腈-甲醇-1%醋酸水溶液(10∶15∶75)为流动相；体积流量为1.0 mL/min；柱温35 ℃；检测波长334 nm。结果 类叶升麻苷在 0.227 2～1.363 2 μg呈良好的线性关系，r=0.999 2，加样回收率、精密度、稳定性和重现性试验结果均符合要求。结论 该方法专属性强、灵敏度高、精确度与重现性好，适用于独一味中类叶升麻苷的测定。

Objective To establish the method for determination of acteoside in Lamiophlomis rotata (Benth.) Kudo. Methods Acteoside was determined by the HPLC with Diamonsil C18 column, the mobile phase consiste of acetonitrilemethanol1% acetic acid(10∶15∶75), flow rate of 1.0 mL/min, UV detection wavelength of 334 nm, and column temperature of 35 ℃. Results This method was linear over ranges of 0.227 2～1.363 2 μg (r=0.999 2) for acteoside. The results of tests for the average recovery, precision, stability, and repeatability accord with the requirements. Conclusion This method is special，ensitive, accurate, and having good repeatability, and can be used for the quality analysis of phenylethanoid glycosides in L. rotata.

“十一五”国家科技支撑计划（2006BAI06A01-02，NO.2007BAI41B06）