目的 研究北冬虫夏草菌丝体醇提物（EECM）对氢化可的松致小鼠肾损作的保护作用，并对其可能的作用机制进行探讨。方法 ICR雄性老龄小鼠随机分为正常组，模型组，EECM低、中、高剂量［0.167、0.334、0.668 g/(kg?d）］组和参仙壮肾胶囊［0.250 g/(kg?d）］组。除正常组和模型组分别给以生理盐水外，其他各组每日一次给以相应的药物，连给30 d；除正常组外，在每次给药后1 h均im氢化可的松25 mg/(kg?d)造模，连给7 d。最后一次给药结束后测定小鼠血清尿素氮（BUN）、肌酐（Scr）、促肾上腺皮质激素（ACTH）和皮质醇（Cor）的水平，并通过光镜观察小鼠肾组织病理变化。结果 与模型组相比，EECM可显著降低模型小鼠肾脏系数、BUN和Scr的量；提高小鼠体质量、血清ACTH和Cor水平；减轻氢化可的松引起的肾脏组织病理损害。结论 EECM对氢化可的松致老龄小鼠肾功能损害具有肾保护作用。
Objective To study the effect of ethanol extract of Cordyceps militaris mycelia (EECM) on hydrocortisoneinduced renal injury in aging mice and the possible mechanisms. Methods Aging male ICR mice were randomly divided into 6 groups, normal guoup, model group, EECM ［0.167、0.334、0.668 g/(kg?d）］ groups, and Shenxian Zhuangshen Capsula ［0.250 g/(kg?d）］ group. All animals except thenormal control and model groups (were given equivalent amount of 0.85% sodium chloride solution) were administrated corresponding drugs once every day, and 30 d continuantly. All groups except the normal control the mice were injected with hydrocortisone 25 mg/(kg?d) at 1 h after administration, and for 7 d. After administration finally, the contents of BUN, Scr, ACTH, Cor in the serum were measured, and the kidney histological changes were observed by optical micorscope. Results Compared with to the model group, the contents of BUN, Scr in the serum, and the coefficent of kidney were obviously decreased in EECM groups； the contents of ACTH and Cor in the serum, and the avoirdupois of mice were obviously increased. Furthermore, EECM ameliorated the kidney pathological changes.Conclusion EECM has protective effects on hydrocortisoneinduced renal lesions in aging mice.