目的 探讨不同溶剂对青龙衣中胡桃醌提取率的影响，进一步通过正交试验优选青龙衣中胡桃醌最佳提取工艺。方法 以胡桃醌的量为评价指标，以乙醇、氯仿、石 油醚、醋酸乙酯为溶剂分别进行超声提取，用HPLC法测定提取物中的胡桃醌，确定出最佳提取溶剂。以溶剂用量(倍)、提取时间(min)、提取次数（次）为考察因素，采用正交试验法L₉(3⁴)确定提取青龙衣中胡桃醌的最佳工艺。结果 最佳提取溶剂为醋酸乙酯，最佳提取工艺条件为A3B3C2，即10倍量的醋酸乙酯，超声2次，每次40 min。结论 用正交试验法优选所得胡桃醌的提取工艺科学合理，适于工业生产。

Objective To explore the effects of different extraction solvents on the yield of juglone in Exocarpium Juglandis Immaturum and further optimize the ex traction technology by orthogonal test. Methods The content of juglone was determined by HPLC and used as the assessment index. Ethanol, benzene, chloroform, and ethyl acetate were selected as the extraction solvents, and extracted by ultrasound wave, respectively, then the optimal extraction conditions were defined by orthogonal design. ResultsThe optimum conditions were: extraction solvent — ethyl acetate (tenfold volumn × twice for 40 m in per time). Conclusion Using the orthogonal test, the optimization of extraction technology for juglone is scientific, rational and can be used in industrial production.

国家自然科学基金项目（30600816）；高等学校博士点学科点专项科研基金（20060240001)