目的 对产于我国甘肃省的中药秦艽的3种基源植物秦艽、麻花秦艽与小秦艽进行RAPD分析,建立具有鉴别意义的DNA指纹图谱.方法 采用RAPD分子标记对秦艽的基因组DNA进行PCR扩增,利用NTSYSpc 2.1和Popgene 3.2软件分析3种秦艽间的亲缘关系,构建树系图.结果 从80条RAPD引物中筛选出4条具有鉴别意义的多态性引物,在3种秦艽中共得到39个DNA条带,其中共有条带2条,多态条带37条.据此获得了能有效区别3种秦艽的多态性RAPD指纹谱.结论 甘肃产秦艽的3种基源植物遗传分化明显,RAPD方法能有效地将它们区分鉴别.

Objective To establish the DNA fingerprints for identification the three origin plants- Gentiana macrophylla,G.strarninea,and G.dahurica of Chinese traditional medicine"Qinjiao"(Radix Gentianae Macrophylla)from Gansu Province.Methods PCR was performed with the genomic DNA of Radix Gentianae Macrophyllae by RAPD marker,the genetic relationship among the Radix Gentianae Macrophylla was analyzed and the DNA molecular dendrogram was set up by the software NTSYSpc 2.1 and Popgene 3.2.Results The four effective p...

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上海市科委资助项目，上海市重点学科建设项目