卢家仕1, 2, 3,卜朝阳1*,吕维莉2,苏建睦3,黄昌艳1,李春牛1.不同产地石斛属种质资源的ISSR遗传多样性分析[J].中草药,2013,44():
不同产地石斛属种质资源的ISSR遗传多样性分析
ISSR analysis on genetic diversity of germplasms resources in Dendrobium SW.from different habitats
  
DOI:
中文关键词:  石斛属  种质资源  ISSR分子标记  遗传多样性  亲缘关系
英文关键词:Dendrobium SW.  germplasms resources  ISSR molecular marker  genetic diversity  genetic relationship
基金项目:农业部热带作物种质资源保护项目(12RZZY-39);广西科技攻关项目(桂科攻1222009-4);广西农科院项目(201014,2011YZ11,2012GW03)
作者单位
卢家仕1, 2, 3,卜朝阳1*,吕维莉2,苏建睦3,黄昌艳1,李春牛1 1. 广西农业科学院花卉研究所广西 南宁 530007 2. 广西农业科学院 广西作物遗传改良生物技术重点开放实验室广西 南宁 530007 3. 广西大学 农学院广西 南宁 530004 
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中文摘要:
      摘 要:目的 对24份不同产地石斛属样品的遗传多样性和亲缘关系进行分析。方法 采用ISSR分子标记技术和非加权平均距离法(UPGMA)对24份石斛属样品进行遗传多样性和聚类分析。结果 从100条ISSR引物中共筛选出6条多态性稳定、清晰的引物, 24份石斛样品共扩增出847个DNA片段,平均每个引物扩增出141个DNA片段,其多态性为100%。结论 24份不同产地石斛样品被划分为6个类群,遗传多样性非常丰富。
英文摘要:
      Abstract: Objective To analyze the genetic diversity and genetic relationship of 24 plant samples in Dendrobium SW. from different habitats. Methods The molecular marker technique of inter-simple sequence repeat (ISSR) and unweighted pair-group method with arithmetic means (UPGMA) were used to study the genetic diversity and cluster analysis of 24 samples. Results Six primers with stable and clear diversity were sieved from 100 ISSR primers. DNA fragments (847) were amplified from 24 samples, and 141 DNA fragments were amplified from each primer in average, with 100% of polymorphic bands. Conclusion The 24 samples in plants of Dendrobium SW. from different habitats are divided into six groups and the genetic diversity is very abundant.
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