目的 采用超高液相色谱四级杆-飞行时间质谱（UPLC-Q-TOF/MS），对活血止痛胶囊化学成分进行快速分析。方法 采用Waters Acquity UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液和0.1%甲酸乙腈为流动相进行梯度洗脱，体积流量0.4 mL/min；分别在正、负离子模式下采集数据，Masslynx 4.1^TM软件分析处理数据。结果 在正、负离子模式下共鉴定出113个化合物，包括萜类、皂苷类、有机酸类、苯酞类和其他类，并对化合物的药材来源进行了归属。结论 建立的方法能够系统、快速、准确地鉴定活血止痛胶囊多种化学成分，本研究为活血止痛胶囊质量评价指标选择及药效物质基础深入研究提供了参考。

Objective To rapidly analyze the chemical constituents of Huoxue Zhitong Capsules performed by ultra-high performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Methods A Waters Acquity UPLC BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm) was used with 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile as a mobile phase. The flow rate was 0.4 mL/min; The samples were collected in positive and negative ion modes, respectively. The processed data was analyzed by Masslynx 4.1^TM software. Results A total of 113 compounds were identified in positive and negative ion mode, including terpenoids, saponins, organic acids, benzoquinones and others, and the medicinal sources of the compounds were assigned. Conclusion The established method can systematically, quickly and accurately identify various chemical components of Huoxue Zhitong Capsule. This study provides a reference for the selection of quality evaluation indicators and the basis of pharmacodynamics of Huoxue Zhitong Capsule.

R284.1

国家自然科学基金重点项目（81830111）；“中医药现代化研究”重点专项资助（2019YFC1711200）