目的 建立常用乌头属藏药材榜那的指纹图谱及多指标成分含量测定方法，为多基原药材的质量标准制定和临床安全用药提供参考。方法 采用HPLC建立榜那药材的指纹图谱并进行相似度评价；测定30批榜那药材中7种生物碱成分含量，并结合主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）评价榜那药材的2种不同基原植物的生物碱成分的差异。结果 建立的指纹图谱确认17个共有峰，指认出12-表-欧乌头碱、雪上一枝蒿庚素、苯甲酰新乌头碱、苯甲酰乌头原碱、新乌头碱、乌头碱、3-乙酰乌头碱7个色谱峰。基于相似度分析，铁棒锤和伏毛铁棒锤种内化学指纹图谱均一，种间指纹图谱存在一定差异。PCA和OPLS-DA结果表明铁棒锤和伏毛铁棒锤2种基原物种在这7种化学成分含量上存在一定差异；且依据OPLS-DA和t检验分析结果可确定伏毛铁棒锤中主要差异成分12-表-欧乌头碱和乌头碱含量显著高于铁棒锤（P<0.01）。结论 本实验所建立的指纹图谱和多组分定量分析方法简便、易操作、信息量大，可用于榜那药材的质量和临床用药安全性控制；有必要建立和完善榜那的双酯型生物碱限度测定。

Objective To establish the chemical fingerprint and multi-index components determination of Tibetan medicine Bangna of Aconitum genus, and provide references for the formulation of quality standards of multi-base original medicinal materials and clinically safe medication. Methods HPLC fingerprint of Bangna was established and evaluated by the similarity evaluation system of TCM. In addition, the content of the seven components of Bangna from 30 batches and the difference of chemical information between the two species of Bangna was investigated by principal component analysis and orthogonal partial least squares discrimination analysis (OPLS-DA) respectively. Results A total of 17 common peaks were identified in the fingerprint that was established by the determination of 30 batches of Bangna, and seven components of which were identified with 12-epi-napelline, songorine, benzoylmesaconitine, benzoylaconine, mesaconitine, aconitine, 3-acetylaconitine. Based on similarity results, the fingerprint had good consistency between the same origin and minor diversity between the different sources. The results of principal component analysis and OPLS-DA showed that there were some differences in the content of seven components between the two species. Based on the results of OPLS-DA and t test, it could be determined that the contents of 12-epi-napelline and aconitine of Aconitum flavum were significantly higher than those in Aconitum pendulum (P<0.01). Conclusion The fingerprint and multi-component quantitative analysis methods were used for the quality and clinically safe medication control of Bangna in this paper is simple, easy to operate, and informative. Moreover, it is necessary to establish and improve the limit determination of diester alkaloids.

R282.2

国家重点研发专项（2018YFC1706200）；全国第四次中药资源普查专项（财社[2017]66号）；四川省重点研发项目（20ZDYF3291）；四川省中医药管理局重点项目（2018C031）