[关键词]
[摘要]
目的 研究蜘蛛香Valeriana jatamansi根及根茎的环烯醚萜成分及其抗流感病毒活性。方法 采用正相硅胶柱色谱、羟丙基葡聚糖凝胶(Sephadex LH-20)和高效液相色谱(HPLC)等色谱方法进行分离纯化,根据理化性质和NMR波谱技术对化合物进行结构鉴定,同时用流感A/WSN/33/2009(H1N1)病毒感染MDCK细胞模型评价化合物的抗流感病毒活性。结果 从蜘蛛香的醋酸乙酯部位中共分离鉴定了20个环烯醚萜类成分,分别鉴定为缬草醛(1)、11-methoxyviburtinal(2)、去酰基缬草醛(3)、异缬草素(4)、去乙酰基异缬草素(5)、10-isovaleroxy-valtrathydrin(6)、jatamanvaltrate Q(7)、缬草醚F(8)、isovaltrate acetoxyhydrin(9)、jatamanvaltrate K(10)、rupesin B(11)、patriscadoid II(12)、jatamanvaltrate W(13)、patriscadoid I(14)、缬草醚D(15)、蜘蛛香素E(16)、jatamanin C(17)、(1S,3R,5S,7S,8S,9S)-1-methoxy-7-hydroxy-8-methyl-3,8-epoxy-Δ4,11-dihyronepetane(18)、(3S,4S,5S,7S,8S,9S)-3,8-ethoxy-7-dihydroxy-4,8-dimethylperhydrocyclopenta[c]pyran(19)、8,9-didehydro-7-hydroxy-dolichodial(20)。结论 化合物5、9、12、14和20为首次从该植物中分离得到。化合物4和9对流感病毒具有抑制作用,半数抑制浓度(IC50)值分别为85.45、19.26 μmol/L。
[Key word]
[Abstract]
Objective To investigate the iridoids from the roots of Valeriana jatamansi and their anti-influenza virus activity. Methods The compounds were isolated and purified over several chromatographic methods, such as silica gel, Sephadex LH-20, HPLC and so on. Their structures were identified by the analysis of their physicochemical properties and NMR data. Their anti-influenza virus activity was evaluated by A/WSN/33/2009 (H1N1) infected MDCK cells. Results Twenty known iridoids (1-20) were isolated from the EtOAc-soluble fraction of 95% EtOH extract of V. jatamansi, and their structures were identified as baldrinal (1), 11-methoxyviburtinal (2), desacylbaldrinal (3), isovaltrate (4), deacetylisovaltrate (5), 10-isovaleroxy-valtrathydrin (6), jatamanvaltrate Q (7), valeriandoid F (8), isovaltrate acetoxyhydrin (9), jatamanvaltrate K (10), rupesin B (11), patriscadoid II (12), jatamanvaltrate W (13), patriscadoid I (14), valeriandoid D (15), valjatrate E (16), jatamanin C (17), (1S,3R,5S,7S,8S,9S)-1-methoxy-7-hydroxy-8-methyl-3,8-epoxy-Δ4,11-dihyronepetane (18), (3S,4S,5S,7S,8S,9S)-3,8-ethoxy-7-dihydroxy-4,8-dimethylperhydrocyclopenta[c] pyran (19) and 8,9-didehydro-7-hydroxy-dolichodial (20). Conclusion Compounds 5, 9, 12, 14, and 20 are isolated from V. jatamansi for the first time. Furthermore, compounds 4 and 9 exhibit anti-influenza A virus activity with IC50 values of 85.45 and 19.26 μmol/L.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金资助项目(31560103)