目的 研究沉香Aquilaria sinensis的倍半萜类化学成分。方法 采用硅胶、ODS、Sephadex LH-20等多种柱色谱及制备型高效液相色谱等现代分离技术对沉香的化学成分进行分离纯化，并根据其理化性质、MS、1D和2D NMR等波谱方法进行结构鉴定；采用96孔板微量稀释法测试了分得的单体化合物对耐药金黄色葡萄球菌的抑菌活性。结果 从沉香95%乙醇提取物中分离得到7个倍半萜类化合物，分别鉴定为（+）-4a，5-二甲基-3-（丙-2-烯基）-八氢萘-2β，8a-二醇（1）、白木香酸（2）、白木香醇（3）、vetaspira-2（11），6-dien-14-al（4）、白木香醛（5）、（-）-10-表-γ-桉叶醇（6）、9β-羟基-α-沉香呋喃（7）。其中化合物1对耐甲氧西林金黄色葡萄球菌的最小抑菌浓度（MIC）为210 μmol/L。结论 化合物1为新的倍半萜类化合物，命名为2β，8aα-二羟基-11-烯-荒漠木烷，其对耐甲氧西林金黄色葡萄球菌有较好的抑制作用。

Objective To investigate the sesquiterpenoid constituents from Aquilaria sinensis. Methods The chemical constituents were isolated and purified by various separation techniques such as silica gel, ODS, Sephadex LH-20, and preparative high- performance liquid chromatography, and their structures were determined according to their physicochemical properties, MS, 1D, and 2D NMR. The antibacterial activity of the obtained compounds against methicillin-resistant Staphylococcus aureus was tested by 96-well plate microdilution method. Results Seven sesquiterpenoids were obtained from 95% ethanol aqueous extract of Aquilaria sinensis and their structures were identified as (+)-4a,5-dimethyl-3-(prop-1-en-2yl)-octahydronaphthalene-2β,8a-diol (1), baimuxinic acid (2), baimuxinol (3), vetaspira-2(11),6-dien-14-al (4), baimuxinal (5), (-)-10-epi-γ-eudesmol (6), and 9β-hydroxyl-α-agarofuran (7). The minimum inhibitory concentration (MIC) of compound 1 against methicillin-resistant Staphylococcus aureus was 210 μmol/L. Conclusion Compound 1 is a new compound named as 2β,8aα-dihydroxy-11-en-eremophilane, which has a good inhibitory effect against methicillin-resistant Staphylococcus aureus.

R284.1

天津市科技重大专项与工程（18ZXXYSY00090）；武警后勤学院创新团队基金项目（WHTD2019）