目的 探讨苦参碱抑制宫颈鳞癌细胞增殖的分子机制。方法 CCK-8法检测苦参碱对宫颈癌细胞SiHa和C33A存活率的影响；流式细胞仪检测细胞周期；高通量测序技术检测苦参碱作用前后宫颈鳞癌细胞中差异表达的RNA。qRT-PCR和Western blotting法检测人脑源性神经营养因子（BDNF）-AS、BDNF mRNA和蛋白在宫颈鳞癌细胞及异体种植瘤组织中的表达。收集2013年3月-2016年12月河南省南阳市第二人民医院32例宫颈鳞状细胞癌组织，运用qRT-PCR法检测宫颈鳞状细胞癌组织中BDNF-AS的表达。结果 苦参碱能够明显抑制宫颈鳞癌细胞的增殖。苦参碱作用前后，共筛选出924个差异表达基因。其中637个（68.9%）基因表达水平上调，287个（31.1%）基因表达水平下调。苦参碱能上调BDNF-AS基因的表达水平。qRT-PCR显示BDNF-AS的表达与宫颈鳞状细胞癌病理分化程度和临床分期之间存在负相关（P<0.05），其表达与预后相关（P=0.04）。Cox回归多因素分析发现BDNF-AS可作为宫颈鳞状细胞癌患者独立的预后因子。结论 BDNF-AS在宫颈鳞状细胞癌的发生发展中可能发挥抑癌作用，苦参碱可能通过上调BDNF-AS抑制宫颈鳞状细胞癌的增殖。

Objective To investigate the mechanism of matrine inhibiting the proliferation of cervical squamous cancer cells. Methods CCK-8 method was used to detect the effect of matrine on the survival rate of SiHa and C33A cells, and flow cytometry was used to detect cell cycle. High throughput sequencing technology was used to screen the differentially expressed genes between control cells and cells being treated with matrine. qRT-PCR and Western blotting method were used to detect the expression of BDNF-AS and BDNF in cervical squamous cancer cells and allograft tumor tissue. A total of 32 cases of cervical squamous cell carcinoma tissues were collected in the Second People's Hospital of Nanyang from March 2013 to December 2016. qRT-PCR was used to detect the expression of BDNF-AS in cervical squamous cell carcinoma tissues of these cases. Results Matrine significantly inhibited the proliferation of cervical squamous carcinoma cells. A total of 924 differentially expressed genes were screened out from cervical squamous carcinoma cells before and after being treated with matrine, 637 (68.9%) were up-regulated while 287 (31.1%) were down-regulated. Matrine up-regulated the expression of BDNF-AS. The expression of BDNF-AS was negative correlated with the degree of pathological differentiation and clinical stage (P<0.05). BDNF-AS expression in tissues was associated with survival of patients with cervical squamous cell carcinoma. Multivariate analyses suggested the expression of BDNF-AS was served as an independent predictor for overall survival. Conclusion BDNF-AS may be a tumor suppressor in the tumorigenesis and tumor progression of cervical squamous cell carcinoma. Matrine may inhibit the proliferation of cervical squamous carcinoma cells by up-regulating the expression of BDNF-AS.

R961.1

河南科技大学博士启动基金（13480044）