[关键词]
[摘要]
目的 探讨佛手散防治帕金森病(PD)的作用及相关机制。方法 体内实验采用利血平模型,观察小鼠肛温和运动不能指标,考察当归挥发油和川芎挥发油及其联合应用的影响;体外实验采用荧光分光光度法,以犬尿胺为底物,检测人单胺氧化酶(hMAO)活性和抑制模式,在此基础上,采用2×4析因设计,探讨当归挥发油与川芎挥发油之间的相互作用。结果 利血平实验发现联合应用当归挥发油和川芎挥发油可显著改善模型小鼠肛温下降和运动不能;酶活性实验发现当归挥发油和川芎挥发油对hMAO-A和hMAO-B均为竞争性抑制作用,两者配伍使用时对hMAO-A和hMAO-B的活性均有显著的协同增效作用。结论 佛手散中的当归、川芎在防治PD中具有显著的协同增效效应,其机制可能与抑制hMAO活性有关。
[Key word]
[Abstract]
Objective To explore the effects and potential mechanism of Foshou Powder on Parkinson's disease. Methods Reserpine was injected in KM mice to establish Parkinson's disease model, and hypothermia and akinesia were measured to evaluate the effects of volatile oil of Angelica sinensis, Ligusticum chuanxiong and combination. The human monoamine oxidase (hMAO) activity and inhibition mode were measured by fluorescence spectrophotometry in vitro with kynuramine as the common substrate of human monoamine oxidase A (hMAO-A) and human monoamine oxidase B (hMAO-B). On this basis, the 2×4 factorial design was applied to research the interaction between Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil. Results The antagonistic experiments of reserpine showed that the application of Angelica sinensis volatile oil and Ligusticum chuanxiong volatile oil significantly improved hypothermia and akinesia. Enzyme activity test revealed that volatile oil of Angelica sinensis and Ligusticum chuanxiong had competitive inhibitory effects on hMAO-A and hMAO-B, and the combination of the two substances had significant synergistic effect on the activity of both hMAO. Conclusion Angelica sinensis and Ligusticum chuanxiong in Foshou Powder have significant synergistic effects in the prevention and treatment of Parkinson's disease, which may be related to the inhibition of MAO activity.
[中图分类号]
R285.5
[基金项目]
四川省科技厅成果转化项目(2018YSZH0012);四川省科技计划项目(2019JDJQ0040)