[关键词]
[摘要]
目的 旨在建立基于体外抗血小板聚集的小金丸生物效价检测方法,评价其质量一致性,筛选小金丸组方中发挥活血作用的中药。方法 小金丸及其组方中的10味中药(人工麝香、木鳖子、制草乌、枫香脂、醋乳香、醋没药、五灵脂、酒当归、地龙、京墨)40%甲醇超声提取,以提取物为供试品通过血小板聚集仪测定其抗血小板聚集率。大鼠腹主动脉取血,制备富血小板血浆(PRP)和乏血小板血浆(PPP),用二磷酸腺苷(ADP)诱导血小板聚集,以阿魏酸钠作为标准对照物质,根据简化概念单位法计算小金丸的抗血小板聚集生物效价。结果 不同厂家、不同批次间小金丸的抗血小板聚集生物效价在0.598~1.338 U/mg。小金丸组方中地龙、五灵脂和木鳖子抑制血小板聚集作用较强,抑制率分别为70.87%、31.83%、67.52%。结论 不同厂家、不同批次小金丸质量一致性较差,地龙、五灵脂和木鳖子可能是小金丸组方中发挥活血作用的关键中药。
[Key word]
[Abstract]
Objective To establish a biological potency assay for Xiaojin Pills against platelet aggregation in vitro, evaluate the quality consistency of Xiaojin Pills, and screen traditioanal Chinese medicines which play the role of promoting blood circulation in Xiaojin Pills. Methods Xiaojin Pills and ten Chinese medicines[artificial musk, Momordica cochinchinensis, Aconitum kusnezoffii, Liquidambar formosana, Boswellia carterii, Commiphora myrrha, Faeces Trogopterori, Angelica sinensis, Pheretima aspergillum, Fragrant Ink] in its formula were extracted by ultrasound in 40% methanol. The antiplatelet aggregation rate of the extract was measured by platelet aggregation meter. The platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared from abdominal aorta of rats. The platelet aggregation was induced by adenosine diphosphate (ADP). With sodium ferulate as a standard reference material, the biological potency of antiplatelet aggregation of Xiaojin Pills was calculated by the simplified probit principle. Results The results showed that the biological potency of Xiaojin Pills was between 0.598 and 1.338 U/mg among different manufacturers and batches. In Xiaojin Pills group, Pheretima, Faeces Trogopterori, and Momordicae Semen had stronger inhibitory effects on platelet aggregation with inhibition rates of 70.87%, 31.83% and 67.52%, respectively. Conclusion The quality consistency of Xiaojin Pills from different manufacturers and batches is poor, and Pheretima, Faeces Trogopterori, and Momordicae Semen may be the key drugs for Xiaojin Pills to play the role of promoting blood circulation.
[中图分类号]
R283.6
[基金项目]
国家自然科学基金资助项目(81873232);国家自然科学基金资助项目(81960696);成都中医药大学中药学学科特色创新科研团队(CXTD2018006)