[关键词]
[摘要]
目的 研究牛蒡Arctium lappa根的化学成分。方法 运用正相硅胶、ODS柱色谱以及半制备型HPLC等手段进行分离纯化,并采用核磁共振等现代波谱学技术鉴定化合物结构。结果 从牛蒡根55%乙醇提取物醋酸乙酯部位中分离得到14个化合物,分别鉴定为1,5-二咖啡酰-3-苹果酰奎尼酸(1)、3,5-二咖啡酰-1-(2-咖啡酰-4-苹果酰)-奎尼酸(2)、3,5-二咖啡酰-1-(2-咖啡酰-4-苹果酰甲酯)-奎尼酸(3)、3,5-二咖啡酰-1-琥珀酰甲酯奎尼酸(4)、3,4-二咖啡酰-1-琥珀酰甲酯奎尼酸(5)、1,3,5-三咖啡酰-4-琥珀酰奎尼酸(6)、1,5-二咖啡酰-3-琥珀酰奎尼酸(7)、1,5-二咖啡酰-4-琥珀酰奎尼酸(8)、1,5-二咖啡酰-4-琥珀酰甲酯奎尼酸(9)、1,5-二咖啡酰-3-琥珀酰甲酯奎尼酸(10)、1,3,4-三咖啡酰奎尼酸(11)、1,4,5-三咖啡酰奎尼酸甲酯(12)、3-咖啡酰奎尼酸(13)、4-羟基苯乙酸(14),其中13个为咖啡酰奎尼酸衍生物。结论 化合物3~5、9、11、12为首次从该属植物中分离得到,化合物14为首次从该植物中分离得到。
[Key word]
[Abstract]
Objective To research the chemical constituents from the roots of Arctium lappa. Methods The compounds were isolated and purified by column chromatography over normal phase silical gel, reverse phase silical gel, ODS column chromatography and semi-preparative HPLC. Their structures were identified by various spectroscopic analysis, including NMR. Results Fourteen compounds were isolated from the 55% EtOH extract of the roots of A. lappa. And their structure were identified as 1,5-di-O-caffeoyl-3-O-maloyl quinic acid (1), 3,5-di-O-caffeoyl-1-O-(2-O-caffeoyl-4-maloyl)-quinic acid (2), 3,5-di-O-caffeoyl-1-(2-O-caffeoyl-4-maloyl methyl ester)-quinic acid (3), 3,5-di-O-caffeoyl-1-O-succinyl methyl ester quinic acid (4), 3,4-di-O-caffeoyl-1-O-succinyl methyl ester quinic acid (5), 1,3,5-tri-O-caffeoyl-4-O-succinyl quinic acid (6), 1,5-di-O-caffeoyl-3-O-succinyl quinic acid (7), 1,5-di-O-caffeoyl-4-O-succinyl quinic acid (8), 1,5-di-O-caffeoyl-4-O-succinyl methyl ester quinic acid (9), 1,5-di-O-caffeoyl-3-O-succinyl methyl ester quinic acid (10), 1,3,4-tri-O-caffeoyl quinic acid (11), 1,4,5-tri-O-caffeoyl quinic acid methyl ester (12), 3-O-caffeoyl quinic acid (13), and 4-hydroxy-phenylacetic acid (14). Conclusion Compounds 3-5, 9, 11、12 are obtained from Arctium genus for the first time, and compound 14 is isolated from A. lappa for the first time.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金资助项目(81673328)