目的 建立15批桃红四物汤汤剂化学指纹图谱及多指标成分含量测定方法，为桃红四物汤经典名方的质量控制及评价提供参考。方法 采用Thermo Hypersil Gold C₁₈色谱柱（250 mm×4.6 mm，5 μm），以甲醇-0.1%磷酸水溶液为流动相，梯度洗脱；体积流量1.0 mL/min，柱温30℃，检测波长225 nm；建立HPLC指纹图谱，采用中药色谱指纹图谱相似度评价系统软件（2012A版）进行相似度分析，结合聚类分析评价15批样品的化学信息差异。进一步通过HPLC多成分波长切换法，测定样品中9种指标成分的含量，采用SIMCA 14.1软件进行偏最小二乘法-判别分析（PLS-DA），以寻找样品间的质量差异成分。结果 建立了15个批次桃红四物汤汤剂HPLC指纹图谱，相似度均>0.96，并确认35个共有锋，指认出没食子酸、绿原酸、苦杏仁苷、芍药内酯苷、羟基红花黄色素A、芍药苷、阿魏酸、洋川芎内酯I、苯甲酰芍药苷、藁本内酯10个色谱峰（分别对应2、8、9、13～16、25、31、32号色谱峰）。含量测定显示，没食子酸、5-羟甲基糠醛、绿原酸、芍药内酯苷、羟基红花黄色素A、芍药苷、阿魏酸、毛蕊花糖苷、洋川芎内酯I 9种成分线性关系良好（r≥0.999 6），含量测定结果分别为187.5～344.4、6.2～154.8、413.2～459.2、507.5～923.5、873.8～1 202.0、2 122.3～2 782.9、59.2～121.3、6.4～26.9、38.9～79.6 μg/g，其中以芍药苷、羟基红花黄色素A、芍药内酯苷等成分含量较高；更进一步将15批不同产地的样品分为3类。通过PLS-DA分析含量测定结果，发现芍药苷、芍药内酯苷、羟基红花黄色素A和5-羟甲基糠醛是影响不同批次桃红四物汤汤剂质量贡献较大的4种成分。结论 HPLC指纹图谱结合多指标成分含量测定方法适用于桃红四物汤复方制剂的质量控制与评价。

Objective To establish chemical fingerprint and multi-components determination of 15 batches of Taohong Siwu Decoction (TSD), and provide reference for the improvement of its quality control. Methods The separation was performed on Thermo Hypersil Gold C₁₈ column (250 mm×4.6 mm, 5 μm) for gradient elution with methanol-0.1% phosphoric acid aqueous solution, flow rate 1.0 mL/min, column temperature 30℃, and detection wavelength 225 nm. The HPLC fingerprint was established and evaluated by the similarity evaluation system of TCM (version 2012A), and the difference of chemical information between 15 batches of different samples was evaluated by cluster analysis. Furthermore, the content of the nine active components in the sample was determined by HPLC multi-component wavelength switching method, with the partial least squares-discriminant analysis (PLS-DA) by SIMCA 14.1 software to find significant components of the quality between the batches. Results The HPLC fingerprint of 15 batches of TSD was established. The similarity was greater than 0.96, and 35 common peaks were identified as gallic acid, chlorogenic acid, amygdalin, albiflorin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, senkyunolide I, benzoylpaeoniflorin and ligustilide (corresponding to peaks 2, 8, 9, 13, 14, 15, 16, 25, 31, and 32). The linearity relationships of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, albiflorin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, verbascoside, and senkyunolide I (r ≥ 0.999 6) were good. The results of content determination respectively were 187.5-344.4, 6.2-154.8, 413.2-459.2, 507.5-923.5, 873.8-1 202.0, 2 122.3-2 782.9, 59.2-121.3, 6.4-26.9, and 38.9-79.6 μg/g, respectively, including higher content of paeoniflorin, hydroxysafflor yellow A, and albiflorin. Furthermore, 15 batches of samples from different origins were classified into three categories. Using PLS-DA analysis, the content determination result showed that paeoniflorin, albiflorin, hydroxysafflor yellow A, and 5-hydroxymethylfurfural were the four components that affected the quality of different batches of TSD. Conclusion HPLC fingerprint combined with multi-components determination is suitable for quality control and evaluation of TSD preparation.

R286.02

四川省重点研发项目（18ZDYF3528）