[关键词]
[摘要]
目的 以纤维蛋白原用量为指标,建立体外定量测定川芎抗凝胶活性的方法,评价川芎及其中成药的抗血栓作用。方法 将川芎先后用乙醇和水定量提取,以总提取物为供试品,测定形成稳定纤维蛋白凝胶时的纤维蛋白原用量。以纤维蛋白原用量为抗凝胶活性的评价指标,用阿魏酸钠标定川芎的抗凝胶活性。根据《中国药典》2015年版(四部)生物检定统计法中的直接测定法,计算川芎的抗凝胶活性。测定了9份川芎药材、饮片及中成药的抗凝胶活性,评价方法的适用性。结果 阿魏酸钠和川芎提取物均具有显著的抗凝胶活性。川芎供试品重复测定抗凝胶活性的RSD值为4.00%(n=6),可信限率为7.82%。不同川芎样品的抗凝胶活性不同,5份川芎药材抗凝胶活性的效价分别为0.72~1.14U/g,川芎饮片及川芎酒炙饮片分别为0.68、1.32 U/g,通脉颗粒和血府逐瘀片分别为2.56、2.51 U/g。结论 建立的方法能准确测定川芎药材、饮片及相关中成药的抗凝胶活性,评价其杭血栓作用。
[Key word]
[Abstract]
Objective Owing to fibrinogen dosage, this study is to develop a method for the bioassay of anti-gel bioactivity of Chuanxiong Rhizoma in vitro, and evaluate the antithrombotic effect of Chuanxiong Rhizoma and its related Chinese patent medicines. Methods Chuanxiong Rhizoma powder was extracted in ethanol and water, respectively. The mixed extract was used as sample to quantify the level of fibrinogen when the stable fibrin gel was formed in vitro. The fibrinogen dosage was chosen as biomarker for anti-gel bioactivity. Sodium ferulate was chosen as reference. The anti-gel bioactivity of Chuanxiong Rhizoma was calculated according to the direct measurement belong to the bioassay statistical method in the Chinese Pharmacopoeia 2015 (Vol. 4). Moreover, the amounts of anti-gel bioactivity were quantified in nine Chuanxiong Rhizoma samples including Chuanxiong Rhizoma raw materials, decoction pieces, and related Chinese patent medicines to evaluate the applicability for this developed method. Results Both sodium ferulate and the extract of Chuanxiong Rhizoma showed significant anti-gel bioactivity (P < 0.01). The RSD for the amounts of anti-gel bioactivity was 4.00% (n=6) in six replicated tests with the confidence limit rate of 7.82% (n=6). The amounts of anti-gel bioactivity were significant difference among the various types of Chuanxiong Rhizoma samples, i.e. 0.72—1.14 U/g for five Chuanxiong Rhizoma raw materials, 0.68 and 1.32 U/g for Chuanxiong Rhizoma decoction pieces and processed slice with yellow wine, 2.56 and 2.51 U/g for Tongmai Granules and Xuefu Zhuyu Tablets. Conclusion The developed method can accurately quantify the level of anti-gel bioactivity in Chuanxiong Rhizoma raw materials, decoction pieces and related Chinese patent medicines to assess their antithrombotic effect.
[中图分类号]
R286.2
[基金项目]
国家自然科学基金资助项目(81773896);四川省重点研发项目(2017SZ0156);四川省教育厅创新团队建设计划(18TD0017)