[关键词]
[摘要]
目的 研究薏苡仁复方对高脂饲料诱导肥胖小鼠的作用及其机制。方法 C57BL/6J雄性小鼠给予正常饲料或高脂饲料喂养。3个月后,除对照组,肥胖模型小鼠(体质量大于对照组小鼠的20%)随机分为模型组,薏苡仁复方低、高剂量(0.59、1.17g/kg)组和阳性对照组(奥利司他,15.6mg/kg)。给药组分别ig给药2个月,对照组和模型组ig同等体积的生理盐水。采用计算机断层扫描(CT)和核磁共振成像(MRI)定量小鼠脂肪含量,组织切片观察脂肪细胞大小,qRT-PCR检测肝脏炎症因子水平。结果 给予薏苡仁复方后,小鼠相对体质量增长率、食物利用率和腰围显著降低(P<0.05)。糖耐量实验说明给予薏苡仁复方可以提高葡萄糖耐受程度(P<0.05)。CT和MRI结果显示,薏苡仁复方组小鼠腹部脂肪厚度和全身脂肪含量均降低,脂肪组织质量和HE染色结果也验证了影像学结果(P<0.05)。同时,给予薏苡仁复方后,肝脏质量降低,脂质堆积减少,而血清三酰甘油(TG)和总胆固醇(TC)水平也显著下降(P<0.05)。此外,薏苡仁复方还可以显著下调血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平和肝组织炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平(P<0.05)。结论 薏苡仁复方通过减少脂质聚集和降低炎症因子水平抵抗高脂饲料诱导的肥胖。
[Key word]
[Abstract]
Objective To study the effects and mechanism of Coicis Semen formula on high-fat diet-induced obesity in mice. Methods C57BL/6J male mice were fed on chow or high-fat diet. After three months, except for control group, the obese mice (body weight more than 20% of the control mice) were randomly divided into model group (model), Coicis Semen formula low-dose group (0.59 g/kg, F-low), Coicis Semen formula high-dose group (1.17 g/kg, F-high) and positive control group (orlistat, 15.6 mg/kg, positive control). F-low, F-high and positive control groups were treated orally for two months, and the control group and the model group were given an equal volume of saline. The fat content of mice was quantified by computed tomography (CT) and magnetic resonance imaging (MRI), the size of adipocytes was observed by tissue staining and the level of inflammatory cytokines was detected by qRT-PCR. Results The results showed that after treatment with Coicis Semen formula, the relative body weight, food utilization ratio and waist circumference were significantly reduced (P < 0.05). An oral glucose tolerance test elucidated that Coicis Semen formula could improve glucose tolerance (P < 0.05). CT and MRI results indicated that the abdominal fat thickness and whole-body fat content of Coicis Semen formula groups were decreased (P < 0.05), which were also confirmed by the adipose tissue weight and HE staining results. Meanwhile, after administration of Coicis Semen formula, the liver weight decreased, less lipid droplets accumulated, and serum triglyceride (TG) and total cholesterol (TC) levels were also significantly lowered (P < 0.05). Coicis Semen formula also significantly down-regulated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and inflammatory factors tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in liver (P < 0.05). Conclusion Coicis Semen formula prevented high-fat diet-induced obesity by reducing lipid aggregation and inflammatory factor expression.
[中图分类号]
R285.5
[基金项目]
国家自然科学基金资助项目(81573013)