目的 研究益智Alpinia oxyphylla仁的化学成分。方法 采用硅胶、MCI柱色谱、Sephadex LH-20、重结晶、半制备液相进行分离纯化，根据理化性质及波谱数据鉴定所得化合物的结构。结果 从益智仁95%乙醇提取物的醋酸乙酯部位分离并鉴定16个化合物，分别鉴定为邻苯二甲酸-双（2'-乙基庚基）酯（1）、（E）-1-（4'-hydroxy-3'-methoxyphenyl）-7-（4″-hydroxy-phenyl）-hept-4-en-3-one（2）、5-hydroxy-7-（4″-hydroxy-3″-methoxyphenyl）-1-phenyl-3-heptanone（3）、1β，4β，7β-trihydroxyeudesmane（4）、bullatantriol（5）、1，5-epoxy-3-hydroxy-1-（4-hydroxy-3，5-dimethoxyphenyl）-7-（4-hydroxy-3-methoxyphenyl） heptanes（6）、1-tetratriacontanol（7）、dihydrogingerenone B（8）、杨芽黄素（9）、白杨素（10）、oxyphyllenone B（11）、（1R，4R，10R）-1β，4α-dihydroxy-11，12，13-trinor-5，6-eudesmen-7-one（12）、胡萝卜苷（13）、1-（4'-羟基苯基）-7-（3″-甲氧基-4″-羟基苯基）-4-烯-3-庚酮（14）、益智酮甲（15）、5-dehydroxy-hexahydro-demethoxycurcuminB（16）。结论 其中化合物1、2、4～8为首次从该属植物中分离得到，化合物3为首次从该植物中分离得到。

Objective To study the chemical constituents of Alpinia oxyphylla. Methods The ethyl acetate fraction of 95% ethanol extract from A. oxphylla was isolated and purified by silica, MCI, Sephadex LH-20 and semi-preparative HPLC, then the structures of obtained compounds were identified by physicochemical properties and spectral data. Results Sixteen compounds were isolated and their chemical structures were identified as phthalic acid-bis (2'-ethyl heptanyl) easter (1), (E)-1-(4'-hydroxy-3'-methoxyphenyl)-7-(4"-hydroxy-phenyl)-hept-4-en-3-one (2), 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone (3), 1β,4β,7β-trihydroxyeudesmane (4), bullatantriol (5), 1,5-epoxy-3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxy-phenyl) heptanes (6), 1-tetratriacontanol (7), dihydrogingerenone B (8), tectochrysin (9), chrysin (10), oxyphyllenone B (11), (1R,4R,10R)-1β,4α-dihydroxy-11,12,13-trinor-5,6-eudesmen-7-one (12), daucosterol (13), 1-(4'-hydroxyphenyl)-7-(3"-methoxy-4"-hydroxyphenyl)-4-ene-3-heptanone (14), yakuchinone A (15) and 5-dehydroxy-hexahydro-demethoxycurcumin B (16). Conclusion A total of 16 compounds were isolated and identified. The compounds 1-2, 4-8 are isolated from the genus for the first time, and compounds 3 were isolated from the plant for the first time.

R284.1

郑州大学第一附属医院青年基金（2015S-02）