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[摘要]
目的 探讨蚕羌、条羌、大头羌的体内外抗炎作用差异,通过谱效关系研究明确羌活抗炎活性物质。方法 采用二甲苯致小鼠耳肿胀急性炎症模型及脂多糖(LPS)诱导小鼠单核巨噬细胞RAW 264.7模型,通过一氧化氮(NO)检测试剂盒与ELISA试剂盒测定炎症因子NO、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的释放量,研究不同商品规格羌活抗炎作用差异;采用灰色关联度分析法对各样品的指纹图谱特征峰峰面积与抗炎活性进行关联度分析。结果 不同商品规格羌活对二甲苯所致小鼠的耳肿胀、炎症因子NO、TNF-α和IL-6的释放均有抑制作用,总体呈现蚕羌 > 大头羌 > 条羌。羌活40个特征峰代表的化学成分与抗炎作用都有关联性,其中17个特征峰与抗炎作用关联性较大,有3个峰为已知成分,分别为绿原酸、阿魏酸和异欧前胡素。结论 蚕羌的抗炎效果最显著,与市场上蚕羌药效最好、价格最高的现状相符,也与传统等级划分标准相一致。初步研究表明羌活抗炎活性物质为绿原酸、阿魏酸和异欧前胡素。
[Key word]
[Abstract]
Objective To explore the difference of anti-inflammatory effects in vitro and in vivo between Canqiang, Tiaoqiang and Datouqiang, and to obtain the anti-inflammatory active substances by relation of fingerprint with pharmacodynamics. Methods Dimethylbenzene for the induction of a mouse ear edema in vivo and LPS-stimulated RAW 264.7 cells in vitro were used to investigate the anti-inflammatory activity, inflammatory factors such as nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined by NO kit and ELISA kit, and the gray correlation analysis method was used to analyze the correlation between the peak area and the anti-inflammatory activity of the fingerprint features of each sample. Results Different commercial specifications presented inhibition on acute inflammation of ear edema and inflammatory factors NO, TNF-α and IL-6, and anti-inflammatory effects were Canqiang > Datouqiang > Tiaoqiang. The chemical composition represented by 40 characteristic peaks was related to anti-inflammatory activity, and 17 characteristic peaks were highly correlated with this effect, among which three peaks were known components, namely chlorogenic acid, ferulic acid and isoimperatorin. Conclusion The anti-inflammatory effect of Canqiang was the most significant, which was consistent with Canqiang being the most expensive and effective in the market, also with traditional grading standards. This study initially obtained anti-inflammatory active substances were chlorogenic acid, ferulic acid and isoimperatorin.
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[基金项目]
国家中药标准化项目:大黄等6种中药饮片标准化建设(ZYBZH-Y-GS-10-B)