目的 比较生姜炮制成干姜前后挥发油透皮吸收促进作用及成分的变化。方法 将生姜炮制成干姜前后提取挥发油，皮肤电阻动力学实验和体外布洛芬透皮实验比较二者的促渗能力，ATR-FTIR红外分析挥发油透皮促渗主要机制，皮肤细胞毒性实验比较了生姜和干姜挥发油的细胞毒性，GC-MS分析成分变化。结果 干姜挥发油的透皮吸收促进作用显著优于生姜挥发油，其透皮促渗机制主要为对皮肤角质层脂质的萃取，而生姜和干姜挥发油的皮肤细胞毒性均显著低于化学促渗剂氮酮，炮制后干姜挥发油的倍半萜类成分比例高于生姜挥发油，透皮后干姜挥发油组皮内的倍半萜类成分比例也高于生姜挥发油组。结论 炮制后的干姜挥发油的透皮吸收作用优于生姜挥发油，验证了中药挥发油透皮吸收促进剂存在"热者易效"的规律。

Objective To compare the penetration-enhancing effect and composition changes of fresh ginger and dried ginger essential oil. Methods The essential oil was extracted before and after fresh ginger being processed into dried ginger, and the penetration-enhancing effect of them was compared by skin electrical resistance and transdermal test of ibuprofen in vitro. The main mechanism of penetration-enhancing effect of the essential oil was studied by ATR-FTIR. The skin cytotoxicity of the essential oils was compared by skin cytotoxicity test, and the composition changes were analyzed by GC-MS. Results Thepenetration-enhancing effect of dried ginger essential oil was much better than the fresh ginger essential oil. The mechanism was mainly due to the skin stratum corneum lipids extraction. The skin cytotoxicity of fresh ginger and dried ginger essential oil was much lower than that of azone. The sesquiterpenes content of dried ginger essential oil was higher than that of fresh ginger essential oil. The intradermal sesquiterpenoids of dried ginger essential oil group after transdermal treatment were higher than those in the fresh ginger essential oil group. Conclusion The transdermal absorption promotion effect of the processed dried ginger essential oil was much better than that of fresh ginger essential oil, which verified the existence of the rule of "hot herbs with better efficacy".

江苏省中药药效与安全性评价重点实验室资助项目（JKLPSE201808）；江苏省高校中药学优势学科建设工程资助项目（PAPD）；江苏省中药资源产业化过程协同创新中心重点项目（ZDXMHT-1-1-5）；江苏省中医药管理局科研专项（ZX2016D1）；江苏省高校优势学科项目（2014）；江苏省高校优势学科专项培育项目（2017）