[关键词]
[摘要]
目的 建立不同产地金银花中7种有机酸、1种黄酮及2种环烯醚萜类成分的含量测定方法。方法 利用UPLC测定不同产地的金银花中10种成分含量。采用Agilent Eclipise Plus C18(50 mm×2.1 mm,1.8 μm)色谱柱,流动相为0.1%磷酸水溶液-甲醇;梯度洗脱,体积流量为0.3 mL/min,柱温30℃。结果 建立了UPLC同时测定金银花药材中7种有机酸、1种黄酮及2种环烯醚萜类成分的方法。采用主成分分析(PCA)及偏最小二乘法(PLS-DA)分析了所收集不同产地金银花10种成分的分布规律及特点,山东、江苏及陕西3个产区各自聚在一大类。结论 该方法稳定、可行,为更全面地评价金银花质量提供参考。
[Key word]
[Abstract]
Objective To establish a method for the determination of seven organic acids, one flavone and two iridoid terpenoids in Lonicerae Japonicae Flos from different habitats. Methods The content of 10 components in Lonicerae Japonicae Flos from different habitats were determined by ultra-performance liquid chromatography (UPLC). The mobile phase for gradient elution was 0.1% phosphoric acid solution (A)-methanol (B); The flow rate was 0.3 mL/min, and the column temperature was 30℃. Results A UPLC method for simultaneous determination of seven organic acids, one flavone and two iridoid terpenoids in Lonicerae Japonicae Flos was established. Principal component analysis (PCA) and partial least squares method (PLS-DA) were used to analyze the distribution and characteristics of 10 constituents of Lonicerae Japonicae Flos collected from different habitats; Three production areas of Shandong, Jiangsu and Shaanxi are respectively grouped into one group. Conclusion The method is stable and feasible, which could be used as a reference for evaluating the quality of Lonicerae Japonicae Flos in a more comprehensive way.
[中图分类号]
R286.2
[基金项目]
国家中医药管理局专项-国家中药标准化项目(ZYBZH-C-JS-31);国家现代农业产业技术体系建设专项资金资助(CARS-21)