目的 建立一种从甘草超滤液中制备甘草素的工艺路线。方法 以甘草素保留率为指标，采用U₅（5³）均匀设计优化超滤工艺；以萃取率为指标，采用Box-Behnken响应面法优化络合萃取工艺；以甘草素反萃取率为指标，确定反萃取甘草素的工艺条件。结果 甘草素的最佳超滤条件为孔径10 nm的无机陶瓷膜、压力0.12 MPa、料液温度25℃，甘草素保留率98.74%；1%三烷基氧化膦（TRPO）、萃取10 min、5 mL有机相溶液为最佳络合萃取条件，甘草素萃取率99.44%；12.5 mmol/L NaOH水溶液为最佳反萃取剂，反萃取率98.88%。结论 作为一种新兴的中药萃取技术，超滤-络合萃取反萃取技术具有高选择、高效、萃取剂循环利用等优点，可为甘草素的研究提供一种全新的制备技术。

Objective A technological route was established for preparing liquiritigenin from the licorice ultrafiltrate. Methods U₅(5³) uniform design was used to optimize the ultrafiltration process with the retention rate of liquiritigenin as the index; Box-Behnken response surface method was used to optimize the complexation extraction process with the extraction rate of liquiritigenin as the index; The technological condition of back extraction of liquiritigenin was determined by the liquiritigenin stripping rate. Results The optimal ultrafiltration conditions for liquiritigenin were inorganic ceramic membranes with a pore size of 10 nm, a pressure of 0.12 MPa, and solution temperature of 25℃, retention rate of liquiritigenin was 98.74%. Optimum complexation extraction conditions were as following:1% TRPO complexing agent, extraction for 10 min in 5 mL organic phase solution, the extraction rate of liquiritigenin was 99.44%; The 12.5 mmol/L NaOH aqueous solution was the best back-extractant, and the back extraction rate was 98.88%. Conclusion As a new Chinese medicine extraction technology, ultrafiltration-complexation extraction and back extraction technology have the advantages of high selectivity, high efficiency, and recycling of extractant, which can provide a new preparation technology for the research of liquiritigenin.

R284.2

国家自然科学基金资助项目（81460608）；甘肃省基础研究创新群体项目（1506RJIA034）