[关键词]
[摘要]
目的 研究海洋特色资源——瘤背石磺Onchidium verruculatum的化学成分。方法 采用硅胶、半制备高效液相色谱、薄层色谱(TLC)检识等方法进行分离纯化,通过NMR、MS等波谱方法对化合物的结构进行鉴定,并结合MTT法测定化合物对人肝癌细胞HepG-2、BEL-7042、人肺腺癌细胞A549和人乳腺癌细胞MCF-7的细胞毒活性。结果 从瘤背石磺的黏液及肌肉组织中分离出1个新的酰胺类化合物,N-(2-羟基-4-甲氧基-5-甲基苯基)甲酰胺(1),化合物1对A549和BEL-7402细胞具有一定的细胞毒活性,半数抑制浓度(IC50)值分别为11.5、19.4 μg/mL。结论 化合物1为1个新的酰胺类化合物,命名为石磺甲酰胺,且对人肿瘤细胞具有一定的细胞毒活性。
[Key word]
[Abstract]
Objective To study the chemical composition of the marine characteristic resource, Onchidium verruculatum from Hainan Island. Methods Compound was separated and purified by silica gel and semi-preparative HPLC, detected by TLC, and identified by NMR and MS data. Compound 1 was evaluated for cytotoxic activity against four kinds of human tumor cell lines HepG-2, BEL-7042, A549, and MCF-7. Results A new amide compound, N-(2-hydroxy-4-methoxy-5-methylphenyl)formamide (1), was isolated from mucus and muscle tissue of O. verruculatum. The results showed that compound 1 has moderate toxic activity against A549 and BEL-7402 cell lines, with IC50 value of 11.5 μg/mL and 19.4 μg/mL. Conclusion Compound 1 is a new amide compound named onchumamide with moderate toxic activity on human tumor cells.
[中图分类号]
R284.1
[基金项目]
海南省自然科学基金面上项目(219MS101);海南省科协青年科技英才创新计划项目(201517)