[关键词]
[摘要]
目的 对黔产景天三七Sedum aizoon的化学成分以及抗氧化活性和α-葡萄糖苷酶抑制活性进行研究。方法 采用多种色谱技术进行分离纯化,根据EI-MS、ESI-MS、1H-和13C-NMR等波谱数据并结合化合物理化性质进行结构鉴定。利用DPPH法筛选所得化合物的抗氧化活性;用PNPG法对所得化合物进行α-葡萄糖苷酶抑制活性的检测。结果 从景天三七的醋酸乙酯部位分离、鉴定得到17个化合物,分别为鸢尾酚酮(1)、山柰酚(2)、槲皮素(3)、杨梅素(4)、鼠李素-3-O-β-D-吡喃葡萄糖(5)、异落叶松脂素-9-O-β-D-吡喃葡萄糖苷(6)、myricitrime(7)、杨梅素-3-O-α-L-阿拉伯糖苷(8)、鸢尾酚酮-2-O-β-D-吡喃葡萄糖(9)、2-O-(trans-caffeoyl)-malic acid 1-methyl ester(10)、2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester(11)、2-O-(trans-caffeoyl)malic acid(12)、对香豆酸(13)、没食子酸乙酯(14)、丁二酸(15)、油酸酰胺(16)、百脉根苷(17)。结论 化合物13、15为首次从景天三七中分离得到,化合物9~12、16为首次从景天属植物中分离得到。化合物2、3、7、8、10、12、14具有良好的抗氧化活性。化合物8有微弱的体外抑制α-葡萄糖苷酶活性,化合物3有较强的体外抑制α-葡萄糖苷酶活性。
[Key word]
[Abstract]
Objective To study the chemical constituents of Sedum aizoon and to screen the anti-oxidant activities and α-glucosidase inhibitory activities of compounds. Methods The compounds were separated and purified by various chromatographic techniques, and their structures were identified by physio-chemical properties and EI-MS, ESI-MS, 1H and 13C NMR. The anti-oxidant activity of compounds was screened by DPPH method. The obtained compounds were subjected to detection of α-glucosidase inhibitory activity by PNPG method. Results Seventeen compounds were isolated from S. aizoon, which were identified as iriflophene (1), kaempferol (2), quercetin (3), myricetin (4), rhamnazin-3-O-β-D-glucopyranoside (5), isolariciresinol-9-O-β-D-glucopyranoside (6), myricitrime (7), myricetin-3-O-α-L-arabinopyranoside (8), iriflophenone-2-O-β-D-glucopyranoside (9), 2-O-(trans-caffeoyl)-malic acid 1-methyl-ester (10), 2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester (11), 2-O-(trans-caffeoyl) malic acid (12), p-coumaric acid (13), ethyl gallate (14), butanedioic acid (15), 9(Z)-octadece-namide (16), and lotaustralin (17). Conclusion Compounds 13 and 15 are isolated from S. aizoon for the first time. Compounds 9-12, and 16 are isolated from genus Sedum for the first time. Compounds 2, 3, 7, 8, 10, 12, and 14 had significant anti-oxidant activity. Compounds 8 showed moderate α-glucosidase inhibitory activity in vitro. Compound 3 showed strong α-glucosidase inhibitory activity in vitro.
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[基金项目]
国家自然科学基金资助项目(31600280);国家自然科学基金资助项目(U1812403);贵州省科技计划项目(黔科合支撑[2018]2799号);黔科合平台人才(2017-5737);省部共建药用植物功效与利用国家重点实验室开放课题项目(FAMP201704K)