[关键词]
[摘要]
目的 建立木香药材UPLC指纹图谱及3,5-O-二咖啡酰基奎宁酸含量测定方法,对不同产地木香的质量进行较全面的评价。方法 采用YMC Trait C18(100 mm×2.1 mm,1.9 μm)色谱柱,以乙腈-0.05%磷酸水溶液为流动相梯度洗脱,指纹图谱检测波长为254 nm,3,5-O-二咖啡酰基奎宁酸含量测定检测波长为327 nm,柱温30℃,体积流量0.30 mL/min。采用相似度评价、聚类分析和主成分分析对13批木香药材指纹图谱进行研究。结果 建立了木香药材的UPLC指纹图谱,确定了8个共有峰;相似度评价、聚类分析和主成分分析结果表明,13批木香药材质量存在差异,不同产地以及同一产地药材均有一定差异。结论 本研究为木香药材的质量控制提供了较为全面、有效的快速评价方法。
[Key word]
[Abstract]
Objective To establish a UPLC fingerprint method and a method for the content determination of 3,5-O-dicaffeoylquinic acid of Aucklandiae Radix, and provide a comprehensive evaluation of the drug from different habitats. Methods UPLC analysis was performed on a YMC Trait C18 (100 mm×2.1 mm, 1.9 μm), with a mobile phase consisting of acetonitrile-0.05% phosphoric acid at the flow rate of 0.30 mL/min. The fingerprint detection wavelength was 254 nm and the content determination detection wavelength of 3,5-O-dicaffeoylquinic acid was 327 nm, meanwhile, the column temperature was controlled at 30℃. Similarity analysis, hierarchical clustering analysis, and principal component analysis were undertaken to investigate the fingerprints of 13 batches of Aucklandiae Radix. Results UPLC fingerprint of Aucklandiae Radix was established and eight common peaks were designated. The results showed that the quality of the batches of samples were not stable. Samples collected from the same region and different regions both had certain differences, as well as the content determination of 3,5-O-dicaffeoylquinic acid. Conclusion The proposed method offered a fast, holistic, and effective method for the quality control of Aucklandiae Radix.
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[基金项目]
广东省省级科技计划项目(科技基础条件建设领域)(2018B030323004)