目的 研究柬埔寨柯拉斯那沉香Aquilaria crassna的化学成分。方法 采用硅胶柱色谱、半制备高效液相色谱等多种色谱技术分离纯化沉香的化学成分，通过波谱解析鉴定化合物结构，并采用MTT法测试化合物对2株人癌细胞株的细胞毒活性。结果 从柯拉斯那沉香乙醇提取物中分离鉴定了4个2-（2-苯乙基）色酮类化合物，分别为6-甲氧基-8-羟基-2-（2-苯乙基）色酮（1）、6-甲氧基-7-羟基-2-[2-（4-甲氧基苯）乙基]色酮（2）、7β，8β-环氧-6α-羟基-5α-甲氧基-5，6，7，8-四氢-2-[2-（4-甲氧基苯）乙基]色酮（3）、rel-（1aR，2R，3R，7bS）-5，6-环氧-7，8-二羟基-5，6，7，8-四氢-2-[2-（4-甲氧基苯）乙基]色酮（4）。结论 化合物1为新化合物，化合物2~4均为首次从柬埔寨柯拉斯那沉香中分离得到，其中化合物4对人胃癌细胞SGC-7901和人肺腺癌细胞A549有较弱的体外抑制活性。

Objective To study the chemical constituents of agarwood originating from Aquilaria crassna in Cambodia. Methods The compounds were isolated and purified by silica gel column chromatography and semi-preparetive HPLC and so on. Their structures were identified by spectroscopic data. All compounds were tested for cytotoxic activities against two human cancer cell lines by MTT method. Results Four compounds were isolated and elucidated as 6-methoxy-8-hydroxy-2-(2-phenylethyl) chromone (1), 6-methoxy-7-hydroxy-2-[2-(4-methoxyphenyl) ethyl] chromone (2), 7β,8β-epoxy-6α-hydroxy-5α-methoxy-5,6,7,8-tetrahydro-2-[2-(4-methoxyphenyl) ethyl] chromone (3), and rel-(1aR,2R,3R,7bS)-5,6-epoxy-7,8-dihydroxy-5,6,7,8-tetrahydro-2-[2-(4-methoxyphenyl) ethyl] chromone (4). Conclusion Compound 1 is a new compound, while compounds 2-4 are isolated from this agarwood for the first time. Among them, compound 4 showed inhibitory activities against SGC-7901 and A549 cells.

海南省自然科学基金创新研究团队项目（2017CXTD020）；中国热带农业科学院院级创新团队（17CXTD-15）；现代农业产业技术体系建设专项资金资助（CARS-21）