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[摘要]
目的 研究不同接种时期对丛枝菌根(AM)真菌侵染的滇重楼幼苗生长发育及重楼皂苷含量的影响,为栽培驯化出高品质的滇重楼奠定基础。方法 采用HPLC测定不同接种时期滇重楼中重楼皂苷I、Ⅱ、VI、VⅡ的含量;采用曲利苯蓝染色法、紫外分光光度法等方法对不同接种时期及不同AM真菌组合的滇重楼的侵染率、生理指标、根茎生物量等进行分析。结果 不同接种时期的AM真菌侵染率较强,保护酶活性、光合色素和可溶性糖含量提高,可溶性蛋白无明显变化,丙二醛含量降低,滇重楼幼苗的抗逆性提高,生长发育良好;栽培种源1年实生苗(2015年8月采收,T7时期)的品质相对较低,栽培种源1年实生苗(2015年6月、7月采收,T5、T6时期)的与栽培种源2年实生苗(2015年8月采收,T8时期)的品质最佳;不同的AM真菌混合组中,S2、S3和S6处理组的效果更佳。结论 接种时期对AMF侵染的滇重楼幼苗生长发育及重楼皂苷含量存在一定的影响。
[Key word]
[Abstract]
Objective To study the effects of different inoculation periods on seedling growth and steroidal saponin content of Paris polyphylla var. yunnanensis, in order to lay a foundation for cultivating and domesticating high-quality Paris polyphylla var. yunnanensis. Methods The content of steroidal saponins I, Ⅱ, VI, and VⅡ in the different vaccination periods of Paris polyphylla var. yunnanensis was determined by HPLC. Meanwhile, trypan blue, UV spectrophotometry and so on were adopted to explore colonization rate, colonization intensity, physiological and biochemical indexes, rhizome biomass of the roots of Paris polyphylla var. yunnanensis. Results Infection rate of AM fungi was higher in different inoculation periods, activities of protective enzymes, photosynthetic pigments and soluble sugars were increased, soluble protein was not changed, content of MDA was decreased, stress resistance of the seedlings of Paris polyphylla var. yunnanensis was improved, and the growth and development were good. Quality of the cultivar one-year seedlings (recovered in August 2015, T7) was relatively low, and cultivar one-year seedlings (harvested in June or July 2015, T5 and T6) and cultivar two years seedlings (recovered in August 2015, T8) were the best. S2, S3 and S6 treatment groups had better effect in different AM fungi mixed groups. Conclusion Seedling growth and steroidal saponin content of Paris polyphylla var. yunnanensis inoculated by different inoculation periods had certain effects.
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[基金项目]
国家自然科学基金资助项目(81260622);重庆市自然科学基金项目资助(cstc2018jcyjAX0267;重庆市自然科学基金项目资助(cstc2015jcyjA10110);云南省教育厅科学研究基金项目资助(2018JS413)